Diversity and spatiotemporal dynamics of bacterial communities : physicochemical and other drivers along an acid mine drainage Volant A. auteur aut IRD Bruneel Odile auteur aut IRD Desoeuvre A. auteur aut IRD Hery M. auteur aut IRD Casiot C. auteur aut IRD Bru N. auteur aut IRD Delpoux S. auteur aut IRD Fahy A. auteur aut IRD Javerliat F. auteur aut IRD Bouchez O. auteur aut IRD Duran R. auteur aut IRD Bertin P. N. auteur aut IRD Elbaz-Poulichet F. auteur aut IRD Lauga B. auteur aut IRD text journalArticle eng text/pdf born digital access Deciphering the biotic and abiotic factors that control microbial community structure over time and along an environmental gradient is a pivotal question in microbial ecology. Carnoules mine (France), which is characterized by acid waters and very high concentrations of arsenic, iron, and sulfate, provides an excellent opportunity to study these factors along the pollution gradient of Reigous Creek. To this end, biodiversity and spatiotemporal distribution of bacterial communities were characterized using T-RFLP fingerprinting and high-throughput sequencing. Patterns of spatial and temporal variations in bacterial community composition linked to changes in the physicochemical conditions suggested that species-sorting processes were at work in the acid mine drainage. Arsenic, temperature, and sulfate appeared to be the most important factors that drove the composition of bacterial communities along this continuum. Time series investigation along the pollution gradient also highlighted habitat specialization for some major members of the community (Acidithiobacillus and Thiomonas), dispersal for Acidithiobacillus, and evidence of extinction/re-thriving processes for Gallionella. Finally, pyrosequencing revealed a broader phylogenetic range of taxa than previous clone library-based diversity. Overall, our findings suggest that in addition to environmental filtering processes, additional forces (dispersal, birth/death events) could operate in AMD community. specialized spatial and temporal dynamics bacterial diversity acid mine drainage arsenic FRANCE 084 062 038 082 Fems Microbiology Ecology 90 1 247-263 2014 0168-6496 https://www.documentation.ird.fr/hor/fdi:010062654 10.1111/1574-6941.12394 0168-6496 [F B010062654] https://www.documentation.ird.fr/hor/fdi:010062654 https://www.documentation.ird.fr/intranet/publi/2014/12/010062654.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-01-12 2017-08-23 fdi:010062654 fre