Humblot Christèle auteur aut IRD Turpin W. auteur aut IRD Chevalier F. auteur aut IRD Picq Christian auteur aut IRD Rochette Isabelle auteur aut IRD Guyot Jean-Pierre auteur aut IRD text journalArticle eng text/pdf born digital access Traditional fermented gruels prepared from cereals are widely used for complementary feeding of young children in Africa and usually have a low energy density. The amylase activity of some lactic acid bacteria (LAB) helps increase the energy content of gruels through partial hydrolysis of starch, thus enabling the incorporation of more starchy material while conserving the desired semi-liquid consistency for young children. Even if this ability is mainly related to the production of alpha-amylase (E.C. 3.2.1.1), in a recent molecular screening, genes coding for enzymes involved in starch metabolism were detected in the efficient amylolytic LAB Lactobacillus plantarum A6: alpha-glucosidase (E.C. 32.1.20), neopullulanase (E.C 3.2.1.135), amylopectin phosphorylase (E.C. 2.4.1.1) and maltose phosphorylase (E.C. 2.4.1.8). The objective of this study was to investigate the expression of these genes in a model of starchy fermented food made from pearl millet (Pennisetum glaucum). Transcriptional and enzymatic analyses were performed during the 18-h fermentation period. Liquefaction was mainly caused by an extracellular alpha amylase encoded by the amyA gene specific to the A6 strain among L. plantarum species and found in both Lactobacillus amylovorus and Lactobacillus manihotivorans. The second most active enzyme was neopullulanase. Other starch metabolizing enzymes were less often detected. The dynamic detection of transcripts of gene during starch fermentation in pearl millet porridge suggests that the set of genes we investigated was not expressed continuously but transiently. specialized mRNA Real time PCR Pearl millet Kinetics BURKINA FASO GHANA AFRIQUE SUBSAHARIENNE 054 185 103-111 2014 0168-1605 https://www.documentation.ird.fr/hor/fdi:010062419 10.1016/j.ijfoodmicro.2014.05.016 0168-1605 [F B010062419] https://www.documentation.ird.fr/hor/fdi:010062419 https://www.documentation.ird.fr/intranet/publi/2014/09/010062419.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-10-02 2017-08-23 fdi:010062419 fre