Ash G.J. auteur aut IRD Lang J.M. auteur aut IRD Triplett L.R. auteur aut IRD Stodart B.J. auteur aut IRD Verdier Valérie auteur aut IRD Cruz C.V. auteur aut IRD Rott P. auteur aut IRD Leach J. E. auteur aut IRD text journalArticle eng text/pdf born digital access The vast amount of data available through next-generation sequencing technology is facilitating the design of diagnostic marker systems. This study reports the use of draft genome sequences from the bacterial plant pathogen Pseudomonas fuscovaginae, the cause of sheath brown rot of rice, to describe the genetic diversity within a worldwide collection of strains representing the species. Based on a comparative analysis with the draft sequences, primers for a loop-mediated isothermal amplification (LAMP) assay were developed to identify P. fuscovaginae. The assay reported here reliably differentiated strains of P. fuscovaginae isolated from rice from a range of other bacteria that are commonly isolated from rice and other plants using a primer combination designated Pf8. The LAMP assay identified P. fuscovaginae purified DNA, live or heat-killed cells from pure cultures, and detected the bacterium in extracts or exudates from infected host plant material. The P. fuscovaginae LAMP assay is a suitable diagnostic tool for the glasshouse and laboratory and could be further developed for in-field surveys. specialized 076 020 084 98 7 909-915 2014 0191-2917 https://www.documentation.ird.fr/hor/fdi:010062381 10.1094/pdis-09-13-0957-re 0191-2917 [F B010062381] https://www.documentation.ird.fr/hor/fdi:010062381 https://www.documentation.ird.fr/intranet/publi/2014/08/010062381.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-09-09 2017-08-23 fdi:010062381 fre