Lang J. M. auteur aut IRD Langlois P. auteur aut IRD Nguyen M. H. R. auteur aut IRD Triplett L. R. auteur aut IRD Purdie L. auteur aut IRD Holton T. A. auteur aut IRD Djikeng A. auteur aut IRD Cruz C. M. V. auteur aut IRD Verdier Valérie auteur aut IRD Leach J. E. auteur aut IRD text journalArticle eng text/pdf born digital access Molecular diagnostics for crop diseases can enhance food security by enabling the rapid identification of threatening pathogens and providing critical information for the deployment of disease management strategies. Loop-mediated isothermal amplification (LAMP) is a PCR-based tool that allows the rapid, highly specific amplification of target DNA sequences at a single temperature and is thus ideal for field-level diagnosis of plant diseases. We developed primers highly specific for two globally important rice pathogens, Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight (BB) disease, and X. oryzae pv. oryzicola, the causal agent of bacterial leaf streak disease (BLS), for use in reliable, sensitive LAMP assays. In addition to pathovar distinction, two assays that differentiate X. oryzae pv. oryzae by African or Asian lineage were developed. Using these LAMP primer sets, the presence of each pathogen was detected from DNA and bacterial cells, as well as leaf and seed samples. Thresholds of detection for all assays were consistently 10(4) to 10(5) CFU ml(-1), while genomic DNA thresholds were between 1 pg and 10 fg. Use of the unique sequences combined with the LAMP assay provides a sensitive, accurate, rapid, simple, and inexpensive protocol to detect both BB and BLS pathogens. specialized AFRIQUE ASIE AMERIQUE 076 084 020 80 15 4519-4530 2014 0099-2240 https://www.documentation.ird.fr/hor/fdi:010062326 10.1128/aem.00274-14 0099-2240 [F B010062326] https://www.documentation.ird.fr/hor/fdi:010062326 https://www.documentation.ird.fr/intranet/publi/2014/08/010062326.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-09-09 2017-08-23 fdi:010062326 fre