@article{fdi:010062326,
  title = {{S}ensitive detection of {X}anthomonas oryzae pathovars oryzae and oryzicola by {L}oop-{M}ediated {I}sothermal {A}mplification},
  author = {{L}ang, {J}. {M}. and {L}anglois, {P}. and {N}guyen, {M}. {H}. {R}. and {T}riplett, {L}. {R}. and {P}urdie, {L}. and {H}olton, {T}. {A}. and {D}jikeng, {A}. and {C}ruz, {C}. {M}. {V}. and {V}erdier, {V}al{\'e}rie and {L}each, {J}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}olecular diagnostics for crop diseases can enhance food security by enabling the rapid identification of threatening pathogens and providing critical information for the deployment of disease management strategies. {L}oop-mediated isothermal amplification ({LAMP}) is a {PCR}-based tool that allows the rapid, highly specific amplification of target {DNA} sequences at a single temperature and is thus ideal for field-level diagnosis of plant diseases. {W}e developed primers highly specific for two globally important rice pathogens, {X}anthomonas oryzae pv. oryzae, the causal agent of bacterial blight ({BB}) disease, and {X}. oryzae pv. oryzicola, the causal agent of bacterial leaf streak disease ({BLS}), for use in reliable, sensitive {LAMP} assays. {I}n addition to pathovar distinction, two assays that differentiate {X}. oryzae pv. oryzae by {A}frican or {A}sian lineage were developed. {U}sing these {LAMP} primer sets, the presence of each pathogen was detected from {DNA} and bacterial cells, as well as leaf and seed samples. {T}hresholds of detection for all assays were consistently 10(4) to 10(5) {CFU} ml(-1), while genomic {DNA} thresholds were between 1 pg and 10 fg. {U}se of the unique sequences combined with the {LAMP} assay provides a sensitive, accurate, rapid, simple, and inexpensive protocol to detect both {BB} and {BLS} pathogens.},
  keywords = {{AFRIQUE} ; {ASIE} ; {AMERIQUE}},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {80},
  numero = {15},
  pages = {4519--4530},
  ISSN = {0099-2240},
  year = {2014},
  DOI = {10.1128/aem.00274-14},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062326},
}