%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gonzalez-Andrade, P.
%A Camara, M.
%A Ilboudo, H.
%A Buheton, B.
%A Jamonneau, Vincent
%A Deborggraeve, S.
%T Diagnosis of trypanosomatid infections : targeting the spliced leader RNA
%D 2014
%L fdi:010062323
%G ENG
%J Journal of Molecular Diagnostics
%@ 1525-1578
%K GUINEE
%M ISI:000338404000004
%N 4
%P 400-404
%R 10.1016/j.jmoldx.2014.02.006
%U https://www.documentation.ird.fr/hor/fdi:010062323
%> https://www.documentation.ird.fr/intranet/publi/2014/08/010062323.pdf
%V 16
%W Horizon (IRD)
%X Trypanosomatids transcribe their genes in Large polycistronic clusters that are further processed into mature mRNA molecules by trans-splicing. During this maturation process, a conserved spliced leader RNA (SL-RNA) sequence of 39 bp is physically linked to the 5' end of the pre-mRNA molecules. Trypanosomatid infections cause a series of devastating diseases in man (sleeping sickness, leishmaniasis, Chagas disease) and animals (nagana, surra, dourine). Here, we investigated the SL-RNA molecule for its diagnostic potential using reverse transcription followed by real-time PCR. As a model, we used Trypanosoma brucei gambiense, which causes sleeping sickness in west and central Africa. We showed that the copy number of the SL-RNA molecule in one single parasitic cell is at least 8600. We observed a lower detection Limit of the SL-RNA assay in spiked blood samples of 100 trypanosomes per milliliter of blood. We also proved that we can detect the trypanosome's SL-RNA in the blood of sleeping sickness patients with a sensitivity of 92% (95% CI, 78%-97%) and a specificity of 96% (95% CI, 86%-99%). The SL-RNA is thus an attractive new molecular target for next-generation diagnostics in diseases caused by trypanosomatids.
%$ 052 ; 020