@article{fdi:010062323,
  title = {{D}iagnosis of trypanosomatid infections : targeting the spliced leader {RNA}},
  author = {{G}onzalez-{A}ndrade, {P}. and {C}amara, {M}. and {I}lboudo, {H}. and {B}uheton, {B}. and {J}amonneau, {V}incent and {D}eborggraeve, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}rypanosomatids transcribe their genes in {L}arge polycistronic clusters that are further processed into mature m{RNA} molecules by trans-splicing. {D}uring this maturation process, a conserved spliced leader {RNA} ({SL}-{RNA}) sequence of 39 bp is physically linked to the 5' end of the pre-m{RNA} molecules. {T}rypanosomatid infections cause a series of devastating diseases in man (sleeping sickness, leishmaniasis, {C}hagas disease) and animals (nagana, surra, dourine). {H}ere, we investigated the {SL}-{RNA} molecule for its diagnostic potential using reverse transcription followed by real-time {PCR}. {A}s a model, we used {T}rypanosoma brucei gambiense, which causes sleeping sickness in west and central {A}frica. {W}e showed that the copy number of the {SL}-{RNA} molecule in one single parasitic cell is at least 8600. {W}e observed a lower detection {L}imit of the {SL}-{RNA} assay in spiked blood samples of 100 trypanosomes per milliliter of blood. {W}e also proved that we can detect the trypanosome's {SL}-{RNA} in the blood of sleeping sickness patients with a sensitivity of 92% (95% {CI}, 78%-97%) and a specificity of 96% (95% {CI}, 86%-99%). {T}he {SL}-{RNA} is thus an attractive new molecular target for next-generation diagnostics in diseases caused by trypanosomatids.},
  keywords = {{GUINEE}},
  booktitle = {},
  journal = {{J}ournal of {M}olecular {D}iagnostics},
  volume = {16},
  numero = {4},
  pages = {400--404},
  ISSN = {1525-1578},
  year = {2014},
  DOI = {10.1016/j.jmoldx.2014.02.006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062323},
}