Merlot S. auteur aut IRD Hannibal Laure auteur aut IRD Martins S. auteur aut IRD Martinelli L. auteur aut IRD Amir H. auteur aut IRD Lebrun Michel auteur aut IRD Thomine S. auteur aut IRD text journalArticle eng text/pdf born digital access We used RNA-Seq to sequence the transcriptome of the nickel hyperaccumulator Psychotria gabriellae endemic from New Caledonia. Using these sequences, we identified the PgIREG1 transporter as a candidate gene for nickel hyperaccumulation.Nickel is an economically important metal and phytotechnologies are being developed to limit the impact of nickel mining on the environment. More than 300 plant species are known to hyperaccumulate nickel. However, our knowledge of the mechanisms involved in nickel accumulation in plants is very limited because it has not yet been possible to study these hyperaccumulators at the genomic level. Here, we used next-generation sequencing technologies to sequence the transcriptome of the nickel hyperaccumulator Psychotria gabriellae of the Rubiaceae family, and used yeast and Arabidopsis as heterologous systems to study the activity of identified metal transporters. We characterized the activity of three metal transporters from the NRAMP and IREG/FPN families. In particular, we showed that PgIREG1 is able to confer nickel tolerance when expressed in yeast and in transgenic plants, where it localizes in the tonoplast. In addition, PgIREG1 shows higher expression in P. gabriellae than in the related non-accumulator species Psychotria semperflorens. Our results designate PgIREG1 as a candidate gene for nickel tolerance and hyperaccumulation in P. gabriellae. These results also show how next-generation sequencing technologies can be used to access the transcriptome of non-model nickel hyperaccumulators to identify the underlying molecular mechanisms. specialized De novo sequencing Ferroportin hyperaccumulator nickel NRAMP Psychotria gabriellae NOUVELLE CALEDONIE 076 020 65 6 1551-1564 2014 0022-0957 https://www.documentation.ird.fr/hor/fdi:010061945 10.1093/jxb/eru025 0022-0957 [F B010061945] https://www.documentation.ird.fr/hor/fdi:010061945 https://www.documentation.ird.fr/intranet/publi/2014/05/010061945.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-05-28 2017-08-23 fdi:010061945 fre