Pawlowiez R. auteur aut IRD Darius H. T. auteur aut IRD Cruchet P. auteur aut IRD Rossi F. auteur aut IRD Caillaud A. auteur aut IRD Laurent Dominique auteur aut IRD Chinain M. auteur aut IRD text journalArticle eng text/pdf born digital access Ciguatera fish poisoning (CFP), a disease caused by consuming fish that have accumulated ciguatoxins (CTXs) in their tissue, is regarded as the most prevalent form of intoxication in French Polynesia. Recently, the Australes, one of the least affected archipelago until the early 1980s, has shown a dramatic increase in its incidence rates in 2009 with unusual CFP cases. In the present work, potential health hazards associated with the proliferation of various marine phytoplankton species and the consumption of fish and marine invertebrates highly popular among local population were assessed in three Australes islands: Raivavae, Rurutu and Rapa. Extracts from the marine dinoflagellates Gambierdiscus, Ostreospis and mat-forming cyanobacteria as well as fish, giant clams and sea urchin samples were examined for the presence of CTXs and palytoxin (PLTX) by using the neuroblastoma cell-based assay (CBA-N2a). Cytotoxic responses observed with both standards (Pacific CTX-3C and PLTX) and targeted marine products indicate that CBA-N2a is a robust screening tool, with high sensitivity and good repeatability and reproducibility. In Rurutu and Raivavae islands, our main findings concern the presence of CTX-like compounds in giant clams and sea urchins, suggesting a second bio-accumulation route for CFP toxins in the ciguatera food chain. In Rapa, the potential CFP risk from Gambierdiscus bloom and fish was confirmed for the first time, with levels of CTXs found above the consumer advisory level of 0.01ng Pacific CTX-1Bg(-1) of flesh in three fish samples. However, despite the presence of trace level of PLTX in Ostreopsis natural assemblages of Rapa, no sign of PLTX accumulation is yet observed in tested fish samples. Because this multi-toxinic context is likely to emerge in most French Polynesian islands, CBA-N2a shows great potential for future applications in the algal- and toxin-based field monitoring programmes currently on hand locally. specialized ciguatera ciguatoxins palytoxin dinoflagellates cyanobacteria fish giant clam sea urchin neuroblastoma cell-based assay French Polynesia risk assessment AUSTRALES ILES POLYNESIE FRANCAISE 054 034 30 3 567-586 2013 1944-0049 https://www.documentation.ird.fr/hor/fdi:010061414 10.1080/19440049.2012.755644 1944-0049 [F B010061414] https://www.documentation.ird.fr/hor/fdi:010061414 https://www.documentation.ird.fr/intranet/publi/2014/01/010061414.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-02-05 2017-08-23 fdi:010061414 fre