@article{fdi:010061363,
  title = {{N}ovel virus discovery and genome reconstruction from field {RNA} samples reveals highly divergent viruses in dipteran hosts},
  author = {{C}ook, {S}. and {C}hung, {B}. {Y}. {W}. and {B}ass, {D}. and {M}oureau, {G}r{\'e}gory and {T}ang, {S}. {Y}. and {M}c{A}lister, {E}. and {C}ulverwell, {C}. {L}. and {G}lucksman, {E}. and {W}ang, {H}. and {B}rown, {T}. {D}. {K}. and {G}ould, {E}. {A}. and {H}arbach, {R}. {E}. and de {L}amballerie, {X}avier and {F}irth, {A}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e investigated whether small {RNA} (s{RNA}) sequenced from field-collected mosquitoes and chironomids ({D}iptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using s{RNA}s sequenced from wild-caught specimens, to inform total {RNA} deep sequencing of samples of particular interest. {U}sing this strategy, we sequenced from adult {A}nopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of {O}chlerotatus caspius and {O}c. detritus mosquitoes, a nearly complete entomobirnavirus genome. {W}e also reconstructed long sequences (1503-6557 nt) related to at least nine other viruses. {C}rucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. {I}t is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.},
  keywords = {{FRANCE} ; {CAMARGUE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {11},
  pages = {e80720},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0080720},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061363},
}