@article{fdi:010061355,
  title = {{A} protocol to improve genotyping of problematic microsatellite loci of {T}rypanosoma brucei gambiense from body fluids},
  author = {{K}abore, {J}. and {D}e {M}eeûs, {T}hierry and {M}ac{L}eod, {A}. and {I}lboudo, {H}. and {C}apewell, {P}. and {C}amara, {M}. and {B}elem, {A}. {M}. {G}. and {B}ucheton, {B}runo and {J}amonneau, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{M}icrosatellite genotyping of {T}rypanosoma brucei gambiense, the causative agent of human {A}frican trypanosomiasis or sleeping sickness, and population genetics tools, are useful for inferring population parameters such as population size and dispersal. {A}mplifying parasite {DNA} directly from body fluids (i.e., blood, lymph or cerebrospinal fluid) allows avoiding costly and tedious isolation phases. {I}t is however associated to increased frequencies of amplification failures (allelic dropouts and/or null alleles) at some loci. {I}n this paper, we present a study focused on three {T}. brucei gambiense microsatellite loci suspected to present amplification problems when amplified from body fluids sampled in {G}uinean sleeping sickness foci. {W}e checked for the real nature of blank and apparent homozygous genotypes of parasite {DNA} directly amplified from body fluids and tested the effect of three different {DNA} quantities of trypanosomes. {O}ur results show that some initially blank and homozygous genotypes happen to be actual heterozygous genotypes. {I}n {G}uinea, lymph from the cervical nymph nodes, known to contain the highest concentrations of parasites, appeared to provide the best amplification results. {S}imply repeating the {PCR} may be enough to retrieve the correct genotype, but we also show that increasing initial {DNA} content provides better results while undertaking first amplification. {W}e finally propose an optimal protocol for amplifying trypanosome's {DNA} directly from body fluids that should be adapted to local characteristics and/or constraints.},
  keywords = {{T}rypanosoma brucei gambiense ; {M}icrosatellite loci ; {B}ody fluids ; {G}enotyping ; {N}ull alleles ; {D}ropouts ; {GUINEE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {20},
  numero = {},
  pages = {171--176},
  ISSN = {1567-1348},
  year = {2013},
  DOI = {10.1016/j.meegid.2013.08.006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061355},
}