Arrieta-Ortiz M. L. auteur aut IRD Rodriguez L. M. auteur aut IRD Perez-Quintero A. L. auteur aut IRD Poulin Lucie auteur aut IRD Diaz A. C. auteur aut IRD Arias Rojas N. auteur aut IRD Trujillo C. auteur aut IRD Restrepo Benavides M. auteur aut IRD Bart R. auteur aut IRD Boch J. auteur aut IRD Boureau T. auteur aut IRD Darrasse A. auteur aut IRD David P. auteur aut IRD Dugué de Bernonville T. auteur aut IRD Fontanilla P. auteur aut IRD Gagnevin L. auteur aut IRD Guérin F. auteur aut IRD Jacques M. A. auteur aut IRD Lauber E. auteur aut IRD Lefeuvre P. auteur aut IRD Medina C. auteur aut IRD Medina E. auteur aut IRD Montenegro N. auteur aut IRD Munoz Bodnar A. auteur aut IRD Noel L. D. auteur aut IRD Ortiz Quinones J. F. auteur aut IRD Osorio D. auteur aut IRD Pardo C. auteur aut IRD Patil P. B. auteur aut IRD Poussier S. auteur aut IRD Pruvost O. auteur aut IRD Robène-Soustrade I. auteur aut IRD Ryan R. P. auteur aut IRD Tabima J. auteur aut IRD Urrego Morales O. G. auteur aut IRD Vernière C. auteur aut IRD Carrere S. auteur aut IRD Verdier Valérie auteur aut IRD Szurek Boris auteur aut IRD Restrepo S. auteur aut IRD Lopez C. auteur aut IRD Koebnik Ralf auteur aut IRD Bernal A. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the infection process of this organism is limited. Previous studies in other bacteria in this genus suggest that advanced draft genome sequences are valuable resources for molecular studies on their interaction with plants and could provide valuable tools for diagnostics and detection. Here we have generated the first manually annotated high-quality draft genome sequence of Xam strain CIO151. Its genomic structure is similar to that of other xanthomonads, especially Xanthomonas euvesicatoria and Xanthomonas citri pv. citri species. Several putative pathogenicity factors were identified, including type III effectors, cell wall-degrading enzymes and clusters encoding protein secretion systems. Specific characteristics in this genome include changes in the xanthomonadin cluster that could explain the lack of typical yellow color in all strains of this pathovar and the presence of 50 regions in the genome with atypical nucleotide composition. The genome sequence was used to predict and evaluate 22 variable number of tandem repeat (VNTR) loci that were subsequently demonstrated as polymorphic in representative Xam strains. Our results demonstrate that Xanthomonas axonopodis pv. manihotis strain CIO151 possesses ten clusters of pathogenicity factors conserved within the genus Xanthomonas. We report 126 genes that are potentially unique to Xam, as well as potential horizontal transfer events in the history of the genome. The relation of these regions with virulence and pathogenicity could explain several aspects of the biology of this pathogen, including its ability to colonize both vascular and non-vascular tissues of cassava plants. A set of 16 robust, polymorphic VNTR loci will be useful to develop a multi-locus VNTR analysis scheme for epidemiological surveillance of this disease. specialized AFRIQUE AMERIQUE DU SUD 076 020 8 11 e79704 2013 1932-6203 https://www.documentation.ird.fr/hor/fdi:010061347 10.1371/journal.pone.0079704 1932-6203 [F B010061347] https://www.documentation.ird.fr/hor/fdi:010061347 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010061347.pdf IRD - Base Horizon / Pleins textes 2014-02-05 2018-06-22 fdi:010061347 fre