Bertin G. I. auteur aut IRD Sabbagh A. auteur aut IRD Guillonneau F. auteur aut IRD Guemouri Sayeh auteur aut IRD Ezinmegnon S. auteur aut IRD Federici C. auteur aut IRD Hounkpatin B. auteur aut IRD Fievet Nadine auteur aut IRD Deloron Philippe auteur aut IRD text journalArticle eng text/pdf born digital access Background. Plasmodium falciparum is responsible for severe malaria, including pregnancy-associated malaria (PAM). During intra-erythrocytic maturation, the infected erythrocyte (iE) membrane is modified by insertion of parasite-derived proteins, primarily consisting of variant surface antigens such as P. falciparum erythrocyte membrane protein-1. Methods. To identify new PAM-specific parasite membrane proteins, we conducted a mass spectrometry-based proteomic study and compared the protein expression profiles of 10 PAM and 10 uncomplicated malaria (UM) samples. Results. We focused on the 454/1139 membrane-associated and hypothetical proteins for comparative analysis. Using filter-based feature-selection methods combined with supervised data analysis, we identified a subset of 53 proteins that distinguished PAM and UM samples. Up to 19/20 samples were correctly assigned to their respective clinical group. A hierarchical clustering analysis of these 53 proteins based on the similarity of their expression profiles revealed 2 main clusters of 40 and 13 proteins that were under-or over-expressed, respectively, in PAM. Conclusions. VAR2CSA is identified and associated with PAM, validating our experimental approach. Other PAM-predictive proteins included PFI1785w, PF14_0018, PFB0115w, PFF0325c, and PFA_0410w. These proteomics data demonstrate the involvement of selected proteins in the pathophysiology of PAM, providing new insights for the definition of potential new targets for a vaccine against PAM. specialized mass spectrometry Plasmodium falciparum pregnancy-associated malaria field isolate protein identification protein abundance BENIN 052 050 208 12 1987-1997 2013 0022-1899 https://www.documentation.ird.fr/hor/fdi:010061336 10.1093/infdis/jit377 0022-1899 [F B010061336] https://www.documentation.ird.fr/hor/fdi:010061336 https://www.documentation.ird.fr/intranet/publi/2013/12/010061336.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-12-23 2017-08-23 fdi:010061336 fre