@article{fdi:010061163,
  title = {{I}n silico identification of a candidate synthetic peptide ({T}sgf1(18-43)) to monitor human exposure to tsetse flies in {W}est {A}frica},
  author = {{D}ama, {E}. and {C}orn{\'e}lie, {S}ylvie and {C}amara, {M}. and {S}omda, {M}. {B}. and {P}oinsignon, {A}nne and {I}lboudo, {H}. and {N}dille, {E}. {E}. and {J}amonneau, {V}incent and {S}olano, {P}hilippe and {R}emou{\'e}, {F}ranck and {B}engaly, {Z}. and {B}elem, {A}. {M}. {G}. and {B}ucheton, {B}runo},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. {H}owever, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. {T}he aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three {G}lossina salivary proteins ({A}da, {A}g5 and {T}sgf1) previously shown to be specifically recognized by plasma from exposed individuals. {M}ethodology/{P}rincipal {F}indings: {S}ynthetic peptides were designed by combining several linear epitope prediction methods and {B}last analysis. {T}he most specific peptides were then tested by indirect {ELISA} on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. {A}nti-{T}sgf1(18-43) specific {I}g{G} levels were low in all three control populations (from rural {A}frica, urban {A}frica and {E}urope) and were significantly higher (p < 0.0001) in the two populations exposed to tsetse flies ({G}uinean {HAT} foci, and {S}outh {W}est {B}urkina {F}aso). {A} positive correlation was also found between {A}nti-{T}sgf1(18-43) {I}g{G} levels and the risk of being infected by {T}rypanosoma brucei gambiense in the sleeping sickness foci of {G}uinea. {C}onclusion/{S}ignificance: {T}he {T}sgf1(18-43) peptide is a suitable and promising candidate to develop a standardize immunoassay allowing large scale monitoring of human exposure to tsetse flies in {W}est {A}frica. {T}his could provide a new surveillance indicator for tsetse control interventions by {HAT} control programs.},
  keywords = {{AFRIQUE} {DE} {L}'{OUEST}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {7},
  numero = {9},
  pages = {e2455 [8 ]},
  ISSN = {1935-2735},
  year = {2013},
  DOI = {10.1371/journal.pntd.0002455},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061163},
}