@article{fdi:010061160,
  title = {{H}uman gut microbiota : repertoire and variations},
  author = {{L}agier, {J}. {C}. and {M}illion, {M}. and {H}ugon, {P}. and {A}rmougom, {F}abrice and {R}aoult, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he composition of human gut microbiota and their relationship with the host and, consequently, with human health and disease, presents several challenges to microbiologists. {O}riginally dominated by culture-dependent methods for exploring this ecosystem, the advent of molecular tools has revolutionized our ability to investigate these relationships. {H}owever, many biases that have led to contradictory results have been identified. {M}icrobial culturomics, a recent concept based on a use of several culture conditions with identification by {MALDI}-{TOF} followed by the genome sequencing of the new species cultured had allowed a complementarity with metagenomics. {C}ulturomics allowed to isolate 31 new bacterial species, the largest human virus, the largest bacteria, and the largest {A}rchaea from human. {M}oreover, some members of this ecosystem, such as {E}ukaryotes, giant viruses, {A}rchaea, and {P}lanctomycetes, have been neglected by the majority of studies. {I}n addition, numerous factors, such as age, geographic provenance, dietary habits, antibiotics, or probiotics, can influence the composition of the microbiota. {F}inally, in addition to the countless biases associated with the study techniques, a considerable limitation to the interpretation of studies of human gut microbiota is associated with funding sources and transparency disclosures. {I}n the future, studies independent of food industry funding and using complementary methods from a broad range of both culture-based and molecular tools will increase our knowledge of the repertoire of this complex ecosystem and host-microbiota mutualism.},
  keywords = {gut microbiota ; culturomics ; metagenomics ; archaea ; transparency ; disclosures ; antibiotics},
  booktitle = {},
  journal = {{F}rontiers in {C}ellular and {I}nfection {M}icrobiology},
  volume = {2},
  numero = {},
  pages = {136 [19 ]},
  ISSN = {2235-2988},
  year = {2012},
  DOI = {10.3389/fcimb.2012.00136},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061160},
}