@article{fdi:010060770,
  title = {{H}igh genetic and pigenetic stability in coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis as revealed by {AFLP}, {MSAP} and the phenotypic variation rate},
  author = {{L}andey, {R}. {B}. and {C}enci, {A}lberto and {G}eorget, {F}. and {B}ertrand, {B}. and {C}amayo, {G}. and {D}echamp, {E}. and {H}errera, {J}. {C}. and {S}antoni, {S}. and {L}ashermes, {P}hilippe and {S}impson, {J}. and {E}tienne, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}mbryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. {E}levated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. {T}his problem could be overcome by using culture conditions that allow moderate cell proliferation. {I}n view of true-to-type large-scale propagation of {C}. arabica hybrids, suspension protocols based on low 2,4-{D} concentrations and short proliferation periods were developed. {A}s mechanisms leading to somaclonal variation are often complex, the phenotypic, genetic and epigenetic changes were jointly assessed so as to accurately evaluate the conformity of suspension-derived plants. {T}he effects of embryogenic suspensions and secondary embryogenesis, used as proliferation systems, on the genetic conformity of somatic embryogenesis-derived plants (emblings) were assessed in two hybrids. {W}hen applied over a 6 month period, both systems ensured very low somaclonal variation rates, as observed through massive phenotypic observations in field plots (0.74% from 200 000 plant). {M}olecular {AFLP} and {MSAP} analyses performed on 145 three year-old emblings showed that polymorphism between mother plants and emblings was extremely low, i.e. ranges of 0-0.003% and 0.07-0.18% respectively, with no significant difference between the proliferation systems for the two hybrids. {N}o embling was found to cumulate more than three methylation polymorphisms. {N}o relation was established between the variant phenotype (27 variants studied) and a particular {MSAP} pattern. {C}hromosome counting showed that 7 of the 11 variant emblings analyzed were characterized by the loss of 1-3 chromosomes. {T}his work showed that both embryogenic suspensions and secondary embryogenesis are reliable for true-to-type propagation of elite material. {M}olecular analyses revealed that genetic and epigenetic alterations are particularly limited during coffee somatic embryogenesis. {T}he main change in most of the rare phenotypic variants was aneuploidy, indicating that mitotic aberrations play a major role in somaclonal variation in coffee.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {2},
  pages = {e56372},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0056372},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060770},
}