Fahmi M. R. auteur aut IRD Solihin D. D. auteur aut IRD Soewardi K. auteur aut IRD Pouyaud Laurent auteur aut IRD Shao Z. J. auteur aut IRD Berrebi P. auteur aut IRD text journalArticle eng text/pdf born digital access A one-step semi-multiplex polymerase chain reaction (PCR) is proposed to distinguish seven species and subspecies of tropical eels, i.e., Anguilla celebesensis, A. interioris, A. nebulosa nebulosa, A. marmorata, A. borneensis, A. bicolor bicolor, and A. bicolor pacifica, known in Indonesian waters. Seven pairs of species-specific primers, including two forward and seven reverse sequences, were designed after alignment of published and new complete mitochondrial cytochrome b (1,140 bp) and 16S ribosomal RNA (rRNA) (1,120 bp) sequences. All species-specific primer pairs are included in one PCR mix, but only one pair of them can amplify a specific fragment from the template DNA that is analyzed. The semi-multiplex PCR amplified a fragment of 230 bp for A. b. bicolor, 372 bp for A. n. nebulosa, 450 bp for A. borneensis, 620 bp for A. marmorata, 670 bp for A. b. pacifica, 720 bp for A. celebesensis, and 795 bp for A. interioris, which are then separated by DNA agarose gel electrophoresis. specialized Semi-multiplex PCR Anguilla spp. Tropical eels Molecular identification Species-subspecies-specific primer INDONESIE 034 020 79 2 185-191 2013 0919-9268 https://www.documentation.ird.fr/hor/fdi:010060763 10.1007/s12562-012-0587-0 0919-9268 [F B010060763] https://www.documentation.ird.fr/hor/fdi:010060763 https://www.documentation.ird.fr/intranet/publi/2013/04/010060763.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-05-02 2017-08-23 fdi:010060763 fre