Abbassi F. auteur aut IRD Raja Z. auteur aut IRD Oury Bruno auteur aut IRD Gazanion Elodie auteur aut IRD Piesse C. auteur aut IRD Sereno Denis auteur aut IRD Nicolas P. auteur aut IRD Foulon T. auteur aut IRD Ladram A. auteur aut IRD text journalArticle eng text/pdf born digital access Temporins are a family of short antimicrobial peptides (8-17 residues) that mostly show potent activity against Gram-positive bacteria. Herein, we demonstrate that temporin-SHd, a 17-residue peptide with a net charge of +2 (FLPAALAGIGGILGKLF(amide)) expressed a broad spectrum of antimicrobial activity. This peptide displayed potent antibacterial activities against Gram-negative and Gram-positive bacteria, including multi-drug resistant Staphylococcus aureus strains, as well as antiparasitic activity against promastigote and the intracellular stage (amastigote) of Leishmania infantum, at concentration not toxic for the macrophages. Temporin-SHd that is structured in a non-amphipathic alpha-helix in anionic membrane-mimetic environments, strongly and selectively perturbs anionic bilayer membranes by interacting with the polar head groups and acyl region of the phospholipids, with formation of regions of two coexisting phases: one phase rich in peptide and the other lipid-rich. The disruption of lipid packing within the bilayer may lead to the formation of transient pores and membrane permeation/disruption once a threshold peptide accumulation is reached. To our knowledge, Temporin-SHd represents the first known 17-residue long temporin expressing such broad spectrum of antimicrobial activity including members of the trypanosomatidae family. Additionally, since only a few shorter members (13 residues) of the temporin family are known to display antileishmanial activity (temporins-TA, -TB and -SHa), SHd is an interesting tool to analyze the antiparasitic mechanism of action of temporins. specialized Antimicrobial peptide Amphibian Temporin-SH Circular dichroism Membrane interaction/permeabilization Antiparasitic activity 020 052 95 2 388-399 2013 0300-9084 https://www.documentation.ird.fr/hor/fdi:010060735 10.1016/j.biochi.2012.10.015 0300-9084 [F B010060735] https://www.documentation.ird.fr/hor/fdi:010060735 https://www.documentation.ird.fr/intranet/publi/2013/04/010060735.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-05-02 2017-08-23 fdi:010060735 fre