@article{fdi:010060520,
  title = {{T}he mi{RNA} and m{RNA} signatures of peripheral blood cells in humans infected with {T}rypanosoma brucei gambiense},
  author = {{L}eong, {S}. and {S}imo, {G}. and {C}amara, {M}. and {J}amonneau, {V}incent and {K}abore, {J}. and {I}lboudo, {H}. and {B}ucheton, {B}runo and {H}oheisel, {J}. {D}. and {C}layton, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}imple, reliable tools for diagnosis of human {A}frican {T}rypanosomiases could ease field surveillance and enhance patient care. {I}n particular, current methods to distinguish patients with (stage {II}) and without (stage {I}) brain involvement require samples of cerebrospinal fluid. {W}e describe here an exploratory study to find out whether mi{RNA}s from peripheral blood leukocytes might be useful in diagnosis of human trypanosomiasis, or for determining the stage of the disease. {U}sing microarrays, we measured mi{RNA}s in samples from {T}rypanosoma brucei gambiense-infected patients (9 stage {I}, 10 stage {II}), 8 seronegative parasite-negative controls and 12 seropositive, but parasite-negative subjects. 8 mi{RNA}s (out of 1205 tested) showed significantly lower expression in patients than in seronegative, parasite-negative controls, and 1 showed increased expression. {T}here were no clear differences in mi{RNA}s between patients in different disease stages. {T}he mi{RNA} profiles could not distinguish seropositive, but parasitologically negative samples from controls and results within this group did not correlate with those from the trypanolysis test. {S}ome of the regulated mi{RNA}s, or their predicted m{RNA} targets, were previously reported changed during other infectious diseases or cancer. {W}e conclude that the changes in mi{RNA} profiles of peripheral blood lymphocytes in human {A}frican trypanosomiasis are related to immune activation or inflammation, are probably disease-non-specific, and cannot be used to determine the disease stage. {T}he approach has little promise for diagnostics but might yield information about disease pathology.},
  keywords = {{AFRIQUE} {DE} {L}'{OUEST} ; {GUINEE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {6},
  pages = {e67312},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0067312},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060520},
}