Dussert Stéphane auteur aut IRD Guérin C. auteur aut IRD Andersson M. auteur aut IRD Joët Thierry auteur aut IRD Tranbarger Timothy auteur aut IRD Pizot Maxime auteur aut IRD Gautier Sarah auteur aut IRD Omore A. auteur aut IRD Durand-Gasselin T. auteur aut IRD Morcillo F. auteur aut IRD text journalArticle eng text/pdf born digital access Oil palm (Elaeis guineensis) produces two oils of major economic importance, commonly referred to as palm oil and palm kernel oil, extracted from the mesocarp and the endosperm, respectively. While lauric acid predominates in endosperm oil, the major fatty acids (FAs) of mesocarp oil are palmitic and oleic acids. The oil palm embryo also stores oil, which contains a significant proportion of linoleic acid. In addition, the three tissues display high variation for oil content at maturity. To gain insight into the mechanisms that govern such differences in oil content and FA composition, tissue transcriptome and lipid composition were compared during development. The contribution of the cytosolic and plastidial glycolytic routes differed markedly between the mesocarp and seed tissues, but transcriptional patterns of genes involved in the conversion of sucrose to pyruvate were not related to variations for oil content. Accumulation of lauric acid relied on the dramatic up-regulation of a specialized acyl-acyl carrier protein thioesterase paralog and the concerted recruitment of specific isoforms of triacylglycerol assembly enzymes. Three paralogs of the WRINKLED1 (WRI1) transcription factor were identified, of which EgWRI1-1 and EgWRI1-2 were massively transcribed during oil deposition in the mesocarp and the endosperm, respectively. None of the three WRI1 paralogs were detected in the embryo. The transcription level of FA synthesis genes correlated with the amount of WRI1 transcripts and oil content. Changes in triacylglycerol content and FA composition of Nicotiana benthamiana leaves infiltrated with various combinations of WRI1 and FatB paralogs from oil palm validated functions inferred from transcriptome analysis. specialized 076 020 162 3 1337-1358 2013 0032-0889 https://www.documentation.ird.fr/hor/fdi:010060484 10.1104/pp.113.220525 0032-0889 [F B010060484] https://www.documentation.ird.fr/hor/fdi:010060484 https://www.documentation.ird.fr/intranet/publi/2013/08/010060484.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-09-03 2020-09-07 fdi:010060484 fre