%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Kaisar, M. M. M.
%A Supali, T.
%A Wiria, A. E.
%A Hamid, F.
%A Wammes, L. J.
%A Sartono, E.
%A Luty, Adrian
%A Brienen, E. A. T.
%A Yazdanbakhsh, M.
%A van Lieshout, L.
%A Verweij, J. J.
%T Epidemiology of Plasmodium infections in Flores Island, Indonesia using real-time PCR
%D 2013
%L fdi:010060415
%G ENG
%J Malaria Journal
%@ 1475-2875
%K Malaria ; Plasmodium falciparum ; Plasmodium vivax ; Plasmodium malariae ; Real-time PCR ; Diagnosis ; Flores
%K INDONESIE ; FLORES
%M ISI:000320043000001
%P 169
%R 10.1186/1475-2875-12-169
%U https://www.documentation.ird.fr/hor/fdi:010060415
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010060415.pdf
%V 12
%W Horizon (IRD)
%X Background: DNA-based diagnostic methods have been shown to be highly sensitive and specific for the detection of malaria. An 18S-rRNA-based, real-time polymerase chain reaction (PCR) was used to determine the prevalence and intensity of Plasmodium infections on Flores Island, Indonesia. Methods: Microscopy and real-time multiplex PCR for the detection of Plasmodium species was performed on blood samples collected in a population-based study in Nangapanda Flores Island, Indonesia. Results: A total 1,509 blood samples were analysed. Real-time PCR revealed prevalence for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae to be 14.5%, 13.2%, and 1.9% respectively. Sub-microscopic parasitaemia were found in more than 80% of all positive cases. The prevalence of P. falciparum and P. vivax was significantly higher in subjects younger than 20 years (p <= 0.01). In the present study, among non-symptomatic healthy individuals, anaemia was strongly correlated with the prevalence and load of P. falciparum infections (p <= 0.01; p = 0.02) and with the load of P. vivax infections (p = 0.01) as detected with real-time PCR. Subjects with AB blood group tend to have a higher risk of being infected with P. falciparum and P. vivax when compared to other blood groups. Conclusion: The present study has shown that real-time PCR provides more insight in the epidemiology of Plasmodium infections and can be used as a monitoring tool in the battle against malaria. The unsurpassed sensitivity of real-time PCR reveals that sub microscopic infections are common in this area, which are likely to play an important role in transmission and control.
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