Auguy Florence auteur aut IRD Fahr M. auteur aut IRD Moulin Esmard Patricia auteur aut IRD Brugel A. auteur aut IRD Laplaze Laurent auteur aut IRD El Mzibri M. auteur aut IRD Filali-Maltouf A. auteur aut IRD Doumas P. auteur aut IRD Smouni A. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Lead is a heavy metal of particular concern with respect to environmental quality and health. The lack of plant species that accumulate and tolerate Pb is a limiting factor to understand the molecular mechanisms involved in Pb tolerance. In this study we identified Hirschfeldia incana, a Brassicaceae collected from metalliferous mine spoils in Morocco, as a Pb accumulator plant. H. incana exhibited high Pb accumulation in mine soils and in hydroponic cultures. Major Pb accumulation occurred in the roots and a part of Pb translocated from the roots to the shoots, even to the siliques. These findings demonstrated that H. incana is a Pb accumulator species. The expression of several candidate genes after Pb-exposure was measured by quantitative PCR and two of them, HiHMA4 and HiMT2a, coding respectively for a P1B-type ATPase and a metallothionein, were particularly induced by Pb-exposure in both roots and leaves. The functional characterization of HiHMA4 and HiMT2a was achieved using Arabidopsis T-DNA insertional mutants. Pb content and primary root growth analysis confirmed the role of these two genes in Pb tolerance and accumulation. H. incana could be considered as a good experimental model to identify genes involved in lead tolerance and accumulation in plants. specialized MAROC 076 084 8 5 art. e61932 2013 1932-6203 https://www.documentation.ird.fr/hor/fdi:010060401 10.1371/journal.pone.0061932 1932-6203 [F B010060401] https://www.documentation.ird.fr/hor/fdi:010060401 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010060401.pdf IRD - Base Horizon / Pleins textes 2013-07-29 2020-06-18 fdi:010060401 fre