Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Plos One art. e61932 MAROC 2013 fdi:010060401 ENG Plos One 1932-6203 ISI:000319654700018 5 10.1371/journal.pone.0061932 https://www.documentation.ird.fr/hor/fdi:010060401 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010060401.pdf 8 Horizon (IRD) Lead is a heavy metal of particular concern with respect to environmental quality and health. The lack of plant species that accumulate and tolerate Pb is a limiting factor to understand the molecular mechanisms involved in Pb tolerance. In this study we identified Hirschfeldia incana, a Brassicaceae collected from metalliferous mine spoils in Morocco, as a Pb accumulator plant. H. incana exhibited high Pb accumulation in mine soils and in hydroponic cultures. Major Pb accumulation occurred in the roots and a part of Pb translocated from the roots to the shoots, even to the siliques. These findings demonstrated that H. incana is a Pb accumulator species. The expression of several candidate genes after Pb-exposure was measured by quantitative PCR and two of them, HiHMA4 and HiMT2a, coding respectively for a P1B-type ATPase and a metallothionein, were particularly induced by Pb-exposure in both roots and leaves. The functional characterization of HiHMA4 and HiMT2a was achieved using Arabidopsis T-DNA insertional mutants. Pb content and primary root growth analysis confirmed the role of these two genes in Pb tolerance and accumulation. H. incana could be considered as a good experimental model to identify genes involved in lead tolerance and accumulation in plants. 076 ; 084 UR232 Maroc