Dama E. auteur aut IRD Cornélie Sylvie auteur aut IRD Somda M. B. auteur aut IRD Camara M. auteur aut IRD Kambire R. auteur aut IRD Courtin Fabrice auteur aut IRD Jamonneau Vincent auteur aut IRD Demettre E. auteur aut IRD Seveno M. auteur aut IRD Bengaly Z. auteur aut IRD Solano Philippe auteur aut IRD Poinsignon Anne auteur aut IRD Remoué Franck auteur aut IRD Belem A. M. G. auteur aut IRD Bucheton Bruno auteur aut IRD text journalArticle eng text/pdf born digital access The saliva of blood sucking arthropods contains a number of pharmacologically active compounds that induce an antibody response in exposed human individuals. The objectives of the present study were (i) to assess the human IgG response directed against salivary antigens of Glossina palpalis gambiensis, the main vector of Trypanosoma brucei gambiense in West Africa, as a biomarker of human tsetse contacts; and (ii) to identify specific salivary antigens. Immune reactivity of human plasma collected within active human African trypanosomiasis (HAT) foci (coastal Guinea), historical foci where tsetse flies are still present (South-West Burkina Faso) and a tsetse free area (Bobo-Dioulasso, Burkina Faso), was measured by ELISA against whole saliva extracts. In the active HAT foci and areas where tsetse flies were present in high densities, specific IgG responses were significantly higher (p < 0.0001) to those in Bobo-Dioulasso or in Loropeni, where tsetse flies were either absent or only present at low densities. Furthermore, 2D-electrophoresis combined with mass spectrometry enabled to reveal that several antigens were specifically recognized by plasma from exposed individuals. Among them, four salivary proteins were successfully identified (Ada, 5'Nuc, Ag5 and Tsgf1). These results represent a first attempt to identify Glossina salivary proteins or synthetic peptides to develop a standardized and specific biomarker of tsetse exposure in West Africa. specialized Human African trypanosomosis Glossina saliva Biomarker Exposure AFRIQUE DE L'OUEST 052 050 15 5 416-427 2013 1286-4579 https://www.documentation.ird.fr/hor/fdi:010060384 10.1016/j.micinf.2013.03.001 1286-4579 [F B010060384] https://www.documentation.ird.fr/hor/fdi:010060384 https://www.documentation.ird.fr/intranet/publi/2013/07/010060384.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-07-29 2017-08-23 fdi:010060384 fre