Yama I. N. auteur aut IRD Garba M. auteur aut IRD Britton-Davidian J. auteur aut IRD Thiberville S. D. auteur aut IRD Dobigny Gauthier auteur aut IRD Gould E. A. auteur aut IRD de Lamballerie Xavier auteur aut IRD Charrel R. N. auteur aut IRD text journalArticle eng text/pdf born digital access The polymerase chain reaction (PCR) has become an essential method for the detection of viruses in tissue specimens. However, it is well known that the presence of PCR inhibitors in tissue samples may cause false-negative results. Hence the identification of PCR inhibitors and evaluation and optimization of nucleic acid extraction and preservation methods is of prime concern in virus discovery programs dealing with animal tissues. Accordingly, to monitor and remove inhibitors we have performed comparative analyses of two commonly used tissue storage methods and five RNA purification techniques using a variety of animal tissues, containing quantified levels of added MS2 bacteriophages as the indicator of inhibition. The results showed (i) no significant difference between the two methods of sample preservation, viz. direct storage at -80 degrees C or 4 degrees C in RNAlater, (ii) lung rodent tissues contained lower levels of inhibitor than liver, kidney and spleen, (iii) RNA extraction using the EZ1 + PK RNA kit was the most effective procedure for removal of RT-PCR inhibitors. specialized Virus screening PCR inhibitors Organ storage Proteinase K Enterobacteria phage MS2 084 020 052 189 2 311-316 2013 0166-0934 https://www.documentation.ird.fr/hor/fdi:010060354 10.1016/j.jviromet.2013.01.024 0166-0934 [F B010060354] https://www.documentation.ird.fr/hor/fdi:010060354 https://www.documentation.ird.fr/intranet/publi/2013/06/010060354.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-07-04 2017-08-23 fdi:010060354 fre