@article{fdi:010060347,
  title = {{P}revalence of molecular markers of drug resistance in an area of seasonal malaria chemoprevention in children in {S}enegal},
  author = {{L}o, {A}. {C}. and {F}aye, {B}. and {B}a, {E}. {H}. and {C}isse, {B}. and {T}ine, {R}. and {A}biola, {A}. and {N}diaye, {M}. and {N}diaye, {J}. {L}. {A}. and {N}diaye, {D}. and {S}okhna, {C}heikh and {G}omis, {J}. {F}. and {D}ieng, {Y}. and {F}aye, {O}. and {N}dir, {O}. and {M}illigan, {P}. and {C}airns, {M}. and {H}allett, {R}. and {S}utherland, {C}. and {G}aye, {O}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {I}n sub-{S}aharan {A}frica, malaria is the leading cause of morbidity and mortality especially in children. {I}n {S}enegal, seasonal malaria chemoprevention ({SMC}) previously referred to as intermittent preventive treatment in children ({IPT}c) is a new strategy for malaria control in areas of high seasonal transmission. {A}n effectiveness study of {SMC}, using sulphadoxine-pyrimethamine ({SP}) plus amodiaquine ({AQ}), was conducted in central {S}enegal from 2008 to 2010 to obtain information about safety, feasibility of delivery, and cost effectiveness of {SMC}. {H}ere are report the effect of {SMC} delivery on the prevalence of markers of resistance to {SP} and {AQ}. {M}ethods: {T}his study was conducted in three health districts in {S}enegal with 54 health posts with a gradual introduction of {SMC}. {T}hree administrations of the combination {AQ} + {SP} were made during the months of {S}eptember, {O}ctober and {N}ovember of each year in children aged less than 10 years living in the area. {C}hildren were surveyed in {D}ecember of each year and samples (filter paper and thick films) were made in 2008, 2009 and 2010. {T}he prevalence of mutations in the pfdhfr, pfdhps, pfmdr1 and pfcrt genes was investigated by sequencing and {RTPCR} in samples positive by microscopy for {P}lasmodium falciparum. {R}esults: {M}utations at codon 540 of pfdhps and codon 164 of pfdhfr were not detected in the study. {A}mong children with parasitaemia at the end of the transmission seasons, the {CVIET} haplotypes of pfcrt and the 86{Y} polymorphism of pfmdr1 were more common among those that had received {SMC}, but the number of infections detected was very low and confidence intervals were wide. {T}he overall prevalence of these mutations was lower in {SMC} areas than in control areas, reflecting the lower prevalence of parasitaemia in areas where {SMC} was delivered. {C}onclusion: {T}he sensitivity of {P}. falciparum to {SMC} drugs should be regularly monitored in areas deploying this intervention. {O}verall the prevalence of genotypes associated with resistance to either {SP} or {AQ} was lower in {SMC} areas due to the reduced number of parasitaemia individuals.},
  keywords = {{P}lasmodium falciparum ; {SMC} ; {S}ulphadoxine-pyrimethamine ; {A}modiaquine ; {P}revalence ; {P}fdhfr ; {P}fdhps ; {P}fcrt ; {P}fmdr1 ; {SENEGAL}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {12},
  numero = {},
  pages = {137},
  ISSN = {1475-2875},
  year = {2013},
  DOI = {10.1186/1475-2875-12-137},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060347},
}