Rey H.Y. auteur aut IRD Faloci M. auteur aut IRD Medina R. auteur aut IRD Dolce N. auteur aut IRD Engelmann Florent auteur aut IRD Mroginski L. auteur aut IRD text journalArticle eng text/pdf born digital access In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles. specialized LEGUMINEUSE CONSERVATION DES RESSOURCES GENETIQUES EMBRYOGENESE SOMATIQUE DESHYDRATATION EXPERIMENTATION IN VITRO ISOENZYME ANALYSE STATISTIQUE ETUDE EXPERIMENTALE ETUDE COMPARATIVE CRYOPRESERVATION ENCAPSULATION SUCROSE STABILITE GENETIQUE TECHNIQUE RAPD 076AMEPLA02 084VITRO 34 6 571-582 2013 0143-2044 https://www.documentation.ird.fr/hor/fdi:010060288 0143-2044 [F B010060288] https://www.documentation.ird.fr/hor/fdi:010060288 https://www.documentation.ird.fr/intranet/publi/depot/2014-01-22/010060288.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2014-01-23 2014-08-25 fdi:010060288 fre