@article{fdi:010060256,
  title = {{R}apid detection and identification of nontuberculous mycobacterial pathogens in fish by using high-resolution melting analysis},
  author = {{P}hung, {T}hu {N}guyet and {C}aruso, {D}omenico and {G}odreuil, {S}. and {K}eck, {N}. and {V}allaeys, {T}. and {A}varre, {J}ean-{C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ycobacterial infections in fish are commonly referred to as piscine mycobacteriosis, irrespectively of the specific identity of the causal organism. {T}hey usually cause a chronic disease and sometimes may result in high mortalities and severe economic losses. {N}early 20 species of {M}ycobacterium have been reported to infect fish. {A}mong them, {M}ycobacterium marinum, {M}. fortuitum, and {M}. chelonae are generally considered the major agents responsible for fish mycobacteriosis. {A}s no quick and inexpensive diagnostic test exists, we tested the potential of high-resolution melting analysis ({HRMA}) to rapidly identify and differentiate several {M}ycobacterium species involved in fish infections. {B}y analyzing both the melting temperature and melting profile of the 16{S}-23{S} r{RNA} internal transcribed spacer ({ITS}), we were able to discriminate 12 different species simultaneously. {S}ensitivity tests conducted on purified {M}. marinum and {M}. fortuitum {DNA} revealed a limit of detection of 10 genome equivalents per reaction. {T}he primers used in this procedure did not lead to any amplification signal with 16 control non-{M}ycobacterium species, thereby demonstrating their specificity for the genus {M}ycobacterium.},
  keywords = {{AQUACULTURE} ; {INFECTION} ; {AGENT} {PATHOGENE} ; {IDENTIFICATION} ; {PCR}.{REACTION} {DE} {POLYMERISATION} {EN} {CHAINE}},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {79},
  numero = {24},
  pages = {7837--7845},
  ISSN = {0099-2240},
  year = {2013},
  DOI = {10.1128/{AEM}.00822-13},
  URL = {https://www.documentation.ird.fr/hor/fdi:010060256},
}