Baronti Cécile auteur aut IRD Pastorino B. auteur aut IRD Charrel R. auteur aut IRD De Lamballerie Xavier auteur aut IRD text journalArticle eng text/pdf born digital access As a partner of the European Virus Archive (EVA) FP7 infrastructure, our research group is maintaining and developing a large virus collection. To meet the standards of the quality management system adopted by all European Virus Archive partners, the detection and eradication of mycoplasma in cell culture supernatants (stored at -80 degrees C or freeze-dried) has to be improved. Although the methods for mycoplasma elimination from infected cell lines were largely described, the decontamination procedures of precious cell culture supernatants was poorly documented. In this study, a large panel of mycoplasma-contaminated virus stocks (enveloped and non enveloped, RNA and DNA viruses) was tested successfully for mycoplasma removal using two simple optimized methods. These easy-to-perform protocols, using respectively Plasmocin (TM) (InvivoGen, Cayla, France) and chloroform, were shown to remove mycoplasma completely from cell supernatant without incidence in viral infectivity. specialized Mycoplasma Treatment Viral culture 020 052 084 187 2 234-237 2013 0166-0934 https://www.documentation.ird.fr/hor/fdi:010059023 10.1016/j.jviromet.2012.09.014 0166-0934 [F B010059023] https://www.documentation.ird.fr/hor/fdi:010059023 https://www.documentation.ird.fr/intranet/publi/2013/03/010059023.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-04-02 2017-08-23 fdi:010059023 fre