@article{fdi:010059023,
  title = {{M}ycoplasma removal : simple curative methods for viral supernatants},
  author = {{B}aronti, {C}{\'e}cile and {P}astorino, {B}. and {C}harrel, {R}. and {D}e {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{A}s a partner of the {E}uropean {V}irus {A}rchive ({EVA}) {FP}7 infrastructure, our research group is maintaining and developing a large virus collection. {T}o meet the standards of the quality management system adopted by all {E}uropean {V}irus {A}rchive partners, the detection and eradication of mycoplasma in cell culture supernatants (stored at -80 degrees {C} or freeze-dried) has to be improved. {A}lthough the methods for mycoplasma elimination from infected cell lines were largely described, the decontamination procedures of precious cell culture supernatants was poorly documented. {I}n this study, a large panel of mycoplasma-contaminated virus stocks (enveloped and non enveloped, {RNA} and {DNA} viruses) was tested successfully for mycoplasma removal using two simple optimized methods. {T}hese easy-to-perform protocols, using respectively {P}lasmocin ({TM}) ({I}nvivo{G}en, {C}ayla, {F}rance) and chloroform, were shown to remove mycoplasma completely from cell supernatant without incidence in viral infectivity.},
  keywords = {{M}ycoplasma ; {T}reatment ; {V}iral culture},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {187},
  numero = {2},
  pages = {234--237},
  ISSN = {0166-0934},
  year = {2013},
  DOI = {10.1016/j.jviromet.2012.09.014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010059023},
}