Haddad Mohamed auteur aut IRD Lelamer Anne-Cécile auteur aut IRD Banuls L. M. Y. auteur aut IRD Vasquez P. auteur aut IRD Carraz Maëlle auteur aut IRD Vaisberg A. auteur aut IRD Castillo D. auteur aut IRD Sauvain Michel auteur aut IRD Rojas R. auteur aut IRD Kiss R. auteur aut IRD text journalArticle eng text/pdf born digital access Two new 13,28-epoxyoleanane triterpene saponins, magnosides A (1) and B (2), were isolated from the 95% ethanolic extract of Cybianthus magnus (Mez) Pipoly roots. Their structures were deduced by a combination of spectral analyses and chemical evidences as compared to data reported in the literature. The hemolytic activity of both compounds was measured. Compound 1 was shown to exhibit the strongest hemolytic activity with a HD50 of 3.8 mu M followed by 2 with a HD50 of 33.5 mu M. The bioactivity of compounds 1 and 2 was also evaluated in vitro against different cellular models including Mycobacterium tuberculosis, Leishmania amazonensis axenic amastigotes, mouse peritoneal macrophages and eight cancer cell lines. While neither of the tested compounds displayed any activity against M. tuberculosis, both exhibited anti-leishmanial activity against axenic amastigotes as well as in vitro growth inhibitory activity against all tested cancer cell lines with IC50 growth inhibitory concentrations ranging between 4 mu M and 33 mu M. The compounds displayed similar growth inhibitory activity in cancer cell lines sensitive to pro-apoptotic stimuli versus those displaying various levels of resistance to such stimuli. Quantitative videomicroscopy analyses revealed that compounds 1 and 2 are cytotoxic. specialized Triterpene saponins Cybianthus magnus Leishmaniasis Cancer In vitro 050 076 020 6 1 128-134 2013 1874-3900 https://www.documentation.ird.fr/hor/fdi:010058991 10.1016/j.phytol.2012.11.011 1874-3900 [F B010058991] https://www.documentation.ird.fr/hor/fdi:010058991 https://www.documentation.ird.fr/intranet/publi/2013/03/010058991.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-04-02 2017-08-23 fdi:010058991 fre