@article{fdi:010058982,
  title = {{G}enomic insights into strategies used by {X}anthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels},
  author = {{P}ieretti, {I}. and {R}oyer, {M}. and {B}arbe, {V}. and {C}arrere, {S}. and {K}oebnik, {R}alf and {C}ouloux, {A}. and {D}arrasse, {A}. and {G}ouzy, {J}. and {J}acques, {M}. {A}. and {L}auber, {E}. and {M}anceau, {C}. and {M}angenot, {S}. and {P}oussier, {S}. and {S}egurens, {B}. and {S}zurek, {B}oris and {V}erdier, {V}al{\'e}rie and {A}rlat, {M}. and {G}abriel, {D}. {W}. and {R}ott, {P}. and {C}ociancich, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {X}anthomonas albilineans causes leaf scald, a lethal disease of sugarcane. {X}. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of {X}anthomonas. {F}or example, this species produces a potent {DNA} gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. {A} first manuscript on the complete genome sequence of the highly pathogenic {X}. albilineans strain {GPE} {PC}73 focused exclusively on distinctive genomic features shared with {X}ylella fastidiosa-another xylem-limited {X}anthomonadaceae. {T}he present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of {X}. albilineans, and to compare, using suppression subtractive hybridization ({SSH}), genomic features of two strains differing in pathogenicity. {R}esults: {C}omparative genomic analyses showed that most of the known pathogenicity factors from other {X}anthomonas species are conserved in {X}. albilineans, with the notable absence of two major determinants of the "artillery" of other plant pathogenic species of {X}anthomonas: the xanthan gum biosynthesis gene cluster, and the type {III} secretion system {H}rp (hypersensitive response and pathogenicity). {G}enomic features specific to {X}. albilineans that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. {SSH} experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. {T}hese 20 genes, which include four {ABC} transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. {W}ith the exception of hypothetical proteins revealed by our comparative genomic analyses and {SSH} experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to {X}. albilineans were identified, supposing that {X}. albilineans has a reduced artillery compared to other pathogenic {X}anthomonas species. {P}articular attention has therefore been given to genomic features specific to {X}. albilineans making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems. {C}onclusions: {T}his study confirms that {X}. albilineans is a highly distinctive species within the genus {X}anthomonas, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {G}enomics},
  volume = {13},
  numero = {},
  pages = {658},
  ISSN = {1471-2164},
  year = {2012},
  DOI = {10.1186/1471-2164-13-658},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058982},
}