@article{fdi:010058973,
  title = {{D}evelopment of a viral biopesticide for the control of the {G}uatemala potato tuber moth {T}ecia solanivora},
  author = {{C}arpio, {C}. and {D}angles, {O}livier and {D}upas, {S}t{\'e}phane and {L}{\'e}ry, {X}avier and {L}opez-{F}erber, {M}. and {O}rbe, {K}. and {P}aez, {D}. and {R}ebaudo, {F}ran{\c{c}}ois and {S}antillan, {A}. and {Y}angari, {B}. and {Z}eddam, {J}ean-{L}ouis},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {G}uatemala potato tuber moth {T}ecia solanivora ({P}ovolny) ({L}ep. {G}elechiidae) is an invasive species from {M}esoamerica that has considerably extended its distribution area in recent decades. {W}hile this species is considered to be a major potato pest in {V}enezuela, {C}olombia, and {E}cuador, currently no specific control methods are available for farmers. {T}o address this issue we developed a biopesticide formulation to be used in integrated pest management of {T}. solanivora, following three steps. {F}irst, search for entomopathogenic viruses were carried out through extensive bioprospections in 12 countries worldwide. {A}s a result, new {P}hthorimaea operculella granulovirus ({P}hop{GV}) isolates were found in {T}. solanivora and five other gelechid species. {S}econd, twenty {P}hop{GV} isolates, including both previously known and newly found isolates, were genetically and/or biologically characterized in order to choose the best candidate for a biopesticide formulation. {S}equence data were obtained for the ecdysteroid {UDP}-glucosyltransferase (egt) gene, a single copy gene known to play a role in pathogenicity. {T}hree different sizes (1086, 1305 and 1353 bp) of egt were found among the virus isolates analyzed. {U}nexpectedly, no obvious correlation between egt size and pathogenicity was found. {B}ioassays on {T}. solanivora neonates showed a maximum of a 14-fold difference in pathogenicity among the eight {P}hop{GV} isolates tested. {T}he most pathogenic {P}hop{GV} isolate, {JLZ}9f, had a medium lethal concentration ({LC}50) of 10 viral occlusion bodies per square mm of consumed tuber skin. {T}hird, we tested biopesticide dust formulations by mixing a dry carrier (calcium carbonate) with different adjuvants (magnesium chloride or an optical brightener or soya lecithin) and different specific amounts of {JLZ}9f. {D}uring laboratory experiments, satisfactory control of the pest (>98% larva mortality compared to untreated control) was achieved with a formulation containing 10 macerated {JLZ}9f-dead {T}. solanivora larvae per kg of calcium carbonate mixed with 50 m{L}/kg of soya lecithin. {T}he final product provides an interesting alternative to chemical pesticides for {A}ndean farmers affected by this potato pest.},
  keywords = {{I}nsect virus ; {B}iological control ; {P}athogenicity ; {V}iral variant ; {E}gt gene ; {ANDES}},
  booktitle = {},
  journal = {{J}ournal of {I}nvertebrate {P}athology},
  volume = {112},
  numero = {2},
  pages = {184--191},
  ISSN = {0022-2011},
  year = {2013},
  DOI = {10.1016/j.jip2012.11.014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058973},
}