Mediannikov Oleg auteur aut IRD Subramanian G. auteur aut IRD Sekeyova Z. auteur aut IRD Bell-Sakyi L. auteur aut IRD Raoult D. auteur aut IRD text journalArticle eng text/pdf born digital access The tick Ixodes ricinus is the most prevalent and widely distributed tick species in Central Europe, commonly found in woodlands, heaths, and forests and particularly abundant in the Alpine region. This tick readily bites humans and transmits a number of bacterial and viral pathogens. We collected 10 live nymphs of I. ricinus ticks from vegetation in the Rovinka forest, Slovakia, and isolated a strain of Arsenophonus nasoniae from one tick using the BME/CTVM2 cell line. A new isolate was then subcultured on axenic media (Columbia agar supplemented with 5% sheep blood). To the best of our knowledge, this bacterium was never previously isolated from hard ticks or identified in ticks in Europe. We amplified and sequenced the 16S rRNA, rpoB, and ftsY genes. Limited genetic characterization showed that the isolated strain is almost identical to a strain from the parasitic wasp Nasonia vitripennis. Electron microscopy revealed a typical morphology of a Gram-negative bacterium, without pill or flagellae. Its role in human and animal pathology remains to be evaluated. specialized Arsenophonus nasoniae Ixodes ricinus Tick cell line Endosymbiont EUROPE 052 3 5-6 366-369 2012 1877-959X https://www.documentation.ird.fr/hor/fdi:010058938 10.1016/j.ttbdis.2012.10.016 1877-959X [F B010058938] https://www.documentation.ird.fr/hor/fdi:010058938 https://www.documentation.ird.fr/intranet/publi/2013/02/010058938.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2013-03-06 2025-06-13 fdi:010058938 fre