@article{fdi:010058938,
  title = {{I}solation of {A}rsenophonus nasoniae from {I}xodes ricinus ticks in {S}lovakia},
  author = {{M}ediannikov, {O}leg and {S}ubramanian, {G}. and {S}ekeyova, {Z}. and {B}ell-{S}akyi, {L}. and {R}aoult, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he tick {I}xodes ricinus is the most prevalent and widely distributed tick species in {C}entral {E}urope, commonly found in woodlands, heaths, and forests and particularly abundant in the {A}lpine region. {T}his tick readily bites humans and transmits a number of bacterial and viral pathogens. {W}e collected 10 live nymphs of {I}. ricinus ticks from vegetation in the {R}ovinka forest, {S}lovakia, and isolated a strain of {A}rsenophonus nasoniae from one tick using the {BME}/{CTVM}2 cell line. {A} new isolate was then subcultured on axenic media ({C}olumbia agar supplemented with 5% sheep blood). {T}o the best of our knowledge, this bacterium was never previously isolated from hard ticks or identified in ticks in {E}urope. {W}e amplified and sequenced the 16{S} r{RNA}, rpo{B}, and fts{Y} genes. {L}imited genetic characterization showed that the isolated strain is almost identical to a strain from the parasitic wasp {N}asonia vitripennis. {E}lectron microscopy revealed a typical morphology of a {G}ram-negative bacterium, without pill or flagellae. {I}ts role in human and animal pathology remains to be evaluated.},
  keywords = {{A}rsenophonus nasoniae ; {I}xodes ricinus ; {T}ick cell line ; {E}ndosymbiont ; {EUROPE} ; {SLOVAQUIE}},
  booktitle = {},
  journal = {{T}icks and {T}ick-{B}orne {D}iseases},
  volume = {3},
  numero = {5-6},
  pages = {366--369},
  ISSN = {1877-959{X}},
  year = {2012},
  DOI = {10.1016/j.ttbdis.2012.10.016},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058938},
}