@article{fdi:010058937,
  title = {{A}pplication of a q{PCR} assay in the investigation of susceptibility to malaria infection of the {M} and {S} molecular forms of {A}n. gambiae s.s. in {C}ameroon},
  author = {{B}oissi{\`e}re, {A}nne and {G}imonneau, {G}eoffrey and {T}chioffo, {M}. {T}. and {A}bate, {L}uc and {B}ayibeki, {A}. and {A}wono-{A}mbene, {P}. {H}. and {N}sango, {S}. {E}. and {M}orlais, {I}sabelle},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-{S}aharan {A}frica. {P}. falciparum is transmitted to the human host by the bite of an {A}nopheles female mosquito, and {A}nopheles gambiae sensus stricto is the most tremendous malaria vector in {A}frica, widespread throughout the afro-tropical belt. {A}n. gambiae s.s. is subdivided into two distinct molecular forms, namely {M} and {S} forms. {T}he two molecular forms are morphologically identical but they are distinct genetically, and differ by their distribution and their ecological preferences. {T}he epidemiological importance of the two molecular forms in malaria transmission has been poorly investigated so far and gave distinct results in different areas. {W}e have developed a real-time quantitative {PCR} (q{PCR}) assay, and used it to detect {P}. falciparum at the oocyst stage in wild {A}n. gambiae s.s. mosquitoes experimentally infected with natural isolates of parasites. {M}osquitoes were collected at immature stages in sympatric and allopatric breeding sites and further infected at the adult stage. {W}e next measured the infection prevalence and intensity in female mosquitoes using the q{PCR} assay and correlated the infection success with the mosquito molecular forms. {O}ur results revealed different prevalence of infection between the {M} and {S} molecular forms of {A}n. gambiae s.s. in {C}ameroon, for both sympatric and allopatric populations of mosquitoes. {H}owever, no difference in the infection intensity was observed. {T}hus, the distribution of the molecular forms of {A}n. gambiae s.s. may impact on the malaria epidemiology, and it will be important to monitor the efficiency of malaria control interventions on the two {M} and {S} forms.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {1},
  pages = {e54820},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0054820},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058937},
}