%0 Book
%9 OS : Ouvrages scientifiques
%A Bakkali, Y.S.E.
%A Duponnois, Robin
%T Natural diversity of ectomycorrhizal fungi in moroccan forest woodlands
%B The mycorrhizal symbiosis in mediterranean environment : importance in ecosystem stability and in soil rehabilitation strategies
%C New York
%D 2012
%E Hafidi, M.
%E Duponnois, Robin
%L fdi:010058484
%G ENG
%I Nova Science
%@ 978-1-62081-278-5
%K REFORESTATION ; INOCULATION ; ECTOMYCORHIZE ; DIVERSITE SPECIFIQUE ; PHENOTYPE ; IDENTIFICATION ; ADN ; ASSOCIATION VEGETALE ; PLANTE PIONNIERE ; PLANTE HOTE ; SYMBIOSE
%K SPECIFICITE ; TECHNIQUE PCR ; TECHNIQUE RFLP
%K MAROC
%K ZONE MEDITERRANNENNE
%P 3-23
%U https://www.documentation.ird.fr/hor/fdi:010058484
%> https://www.documentation.ird.fr/intranet/publi/2023-12/010058484.pdf
%W Horizon (IRD)
%X In Morocco, the forest woodlands undergo a worrying regression in spite of the intensive plantation programs. The success of these programs with a good plant establishment after transfer to the field is related among others to the cultural techniques used in nurseries. The controlled mycorrhization in nurseries, by selected ectomycorrhizal fungi, improves survival, establishment, and growth of seedlings after out planting. The aboveground surveys of sporocarps are usually poor indicators of the community structure below ground. This is because sporocarp production is triggered by specific environmental conditions. A classical approach for identifying mycorrhizas is therefore to trace hyphal connections between sporocarps and mycorrhizal sheaths. However, special skills are required when root density is so high that hyphae emerging from the stipe base cannot be attributed unambiguously to a single mycorrhizal. An alternative promising way to identify mycorrhizas consists of comparing specific DNA regions of mycorrhizas and sporocarps. Polymerase Chain Reaction coupled with Restriction Fragment Length Polymorphism analyses (PCR/RFLP) and sequencing have been applied in mycorrhizal research to identify strains of naturally occurring mycorrhizal fungi and also to differentiate and identify mycorrhizal symbionts unambiguously. The main objective of this chapter is to present and discuss some of the relevant research work on ectomycorrhizal fungi diversity in Moroccan forest ecosystems to select fungal isolates and producing fungal inocula adapted to local conditions in controlled mycorrhization programs.
%B Environmental Science, Engineering and Technology
%$ 084SYMBIO03 ; 082VEGET02