@book{fdi:010058484, title = {{N}atural diversity of ectomycorrhizal fungi in moroccan forest woodlands}, author = {{B}akkali, {Y}.{S}.{E}. and {D}uponnois, {R}obin}, editor = {}, language = {{ENG}}, abstract = {{I}n {M}orocco, the forest woodlands undergo a worrying regression in spite of the intensive plantation programs. {T}he success of these programs with a good plant establishment after transfer to the field is related among others to the cultural techniques used in nurseries. {T}he controlled mycorrhization in nurseries, by selected ectomycorrhizal fungi, improves survival, establishment, and growth of seedlings after out planting. {T}he aboveground surveys of sporocarps are usually poor indicators of the community structure below ground. {T}his is because sporocarp production is triggered by specific environmental conditions. {A} classical approach for identifying mycorrhizas is therefore to trace hyphal connections between sporocarps and mycorrhizal sheaths. {H}owever, special skills are required when root density is so high that hyphae emerging from the stipe base cannot be attributed unambiguously to a single mycorrhizal. {A}n alternative promising way to identify mycorrhizas consists of comparing specific {DNA} regions of mycorrhizas and sporocarps. {P}olymerase {C}hain {R}eaction coupled with {R}estriction {F}ragment {L}ength {P}olymorphism analyses ({PCR}/{RFLP}) and sequencing have been applied in mycorrhizal research to identify strains of naturally occurring mycorrhizal fungi and also to differentiate and identify mycorrhizal symbionts unambiguously. {T}he main objective of this chapter is to present and discuss some of the relevant research work on ectomycorrhizal fungi diversity in {M}oroccan forest ecosystems to select fungal isolates and producing fungal inocula adapted to local conditions in controlled mycorrhization programs.}, keywords = {{REFORESTATION} ; {INOCULATION} ; {ECTOMYCORHIZE} ; {DIVERSITE} {SPECIFIQUE} ; {PHENOTYPE} ; {IDENTIFICATION} ; {ADN} ; {ASSOCIATION} {VEGETALE} ; {PLANTE} {PIONNIERE} ; {PLANTE} {HOTE} ; {SYMBIOSE} ; {SPECIFICITE} ; {TECHNIQUE} {PCR} ; {TECHNIQUE} {RFLP} ; {MAROC} ; {ZONE} {MEDITERRANNENNE}}, address = {{N}ew {Y}ork}, publisher = {{N}ova {S}cience}, series = {{E}nvironmental {S}cience, {E}ngineering and {T}echnology}, pages = {3-23}, year = {2012}, ISBN = {978-1-62081-278-5}, URL = {https://www.documentation.ird.fr/hor/fdi:010058484}, }