@article{fdi:010058168,
  title = {{G}eographical gradients in the genome size variation of wild coffee trees ({C}offea) native to {A}frica and {I}ndian {O}cean islands},
  author = {{R}azafinarivo, {N}orosoa and {R}akotomalala, {J}.{J}. and {B}rown, {S}.{C}. and {B}ourge, {M}. and {H}amon, {S}erge and {K}ochko, {A}lexandre de and {P}oncet, {V}al{\'e}rie and {D}ubreuil {T}ranchant, {C}hristine and {C}outuron, {E}mmanuel and {G}uyot, {R}omain and {H}amon, {P}erla},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he genus {C}offea, mainly native to {A}frica and to the {I}ndian {O}cean islands ({M}ascarocoffea), accounts for 124 species. {G}enome size data are available for 23 {A}frican species. {T}he aim of this study was to assess the genome size of 44 {M}ascarocoffea species and to investigate possible association with species geographic distribution, stomata traits, and species relationships. 2 {C} values were measured using flow cytometry. {A} lyophilization procedure for leaves was tested. {T}he 2 {C} nuclear {DNA} content of {M}ascarocoffea species ranged from 0.96 to 1.41 pg. {C}offea mauritiana and {C}offea humblotiana have the smallest genomes and {C}offea millotii has the largest. {M}ean 2 {C} {DNA} for {M}ascarocoffea and {A}frica is 1.19 and 1.43 pg, respectively. {T}he overall {DNA} values corresponded to two partially overlapped normal distributions: one harboring species from east {A}frica {M}ascarocoffea, the other harboring species from west/central {A}frica. {P}lotted on a geographical map according to the native origin of species, these values showed a gradient in {M}adagascar and {A}frica. {G}enome sizes increased following a north to southeast gradient in {M}adagascar and an east to west gradient in {A}frica. {N}one, or only weak correlations were noted between genome size and stomata parameters. {G}enetically close species could be highly distinctive in their genome size while divergent species could be similarly sized. {T}he non-random geographic distribution and habitat of species, and the absence of correlation between genome size and genetic relationships, suggest that during {C}offea genome evolution, both {DNA} content increase and/or decrease occurred independently in {A}frica and in the {I}ndian {O}cean {I}slands.},
  keywords = {{C}offea ; {M}adagascar ; {A}frica ; {G}enome size variation ; {F}low cytometry},
  booktitle = {},
  journal = {{T}ree {G}enetics and {G}enomes},
  volume = {8},
  numero = {6},
  pages = {1345--1358},
  ISSN = {1614-2942},
  year = {2012},
  DOI = {10.1007/s11295-012-0520-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010058168},
}