@article{fdi:010057521,
  title = {{G}enetic diversity and distribution of {B}radyrhizobium and {A}zorhizobium strains associated with the herb legume {Z}ornia glochidiata sampled from across {S}enegal},
  author = {{G}ueye, {F}atou and {M}oulin, {L}ionel and {S}ylla, {S}. and {N}doye, {I}. and {B}{\'e}na, {G}illes},
  editor = {},
  language = {{ENG}},
  abstract = {{H}erb legumes have great potential for rehabilitation of semi-arid degraded soils in {S}ahelian ecosystems as they establish mutualistic symbiosis with {N}-2-fixing rhizobia. {A} phylogenetic analysis was performed for 78 root nodule bacteria associated with the common {S}ahelian herb legume {Z}ornia glochidiata {R}eichb ex {DC} in {S}enegal. {B}ased on {ITS} (r{DNA}16{S}-23{S}) and rec{A} sequences, these strains were shown to belong to the two genera {B}radyrhizobium? and {A}zorhizobium. {S}trains of this latter, although frequent, formed small and ineffective nodules and suggested a parasitism rather than a symbiotic association. {A} potential negative effect of {A}zorhizobium on {Z}ornia growth was tested for when inoculated alone or in association with a {B}radyrhizobium strain. {B}radyrhizobium isolates were distributed in four groups. {G}roups {A} and {B} were two sister clades in a larger monophyletic group also including {B}radyrhizobium liaoningense, {B}radyrhizobium yuanmingense, and {B}radyrhizobium japonicum. {S}trains of cluster {D} fell in a sister clade of the photosynthetic {B}radyrhizobium sp. group, including {ORS}278, whereas group {C} appeared to be divergent from all known {B}radyrhizobium {C}lusters. {A}mplified fragment length polymorphism ({AFLP}) clustering was congruent with {ITS} and rec{A} phylogenies, but displayed much more variability. {H}owever, within the main {B}radyrhizobium clades, no obvious relationship could be detected between clustering and geographical origin of the strains. {E}ach sub-cluster included strains sampled from different locations. {C}onversely, {A}zorhizobium strains showed a tendency in the phylogeny to group together according to the site of sampling. {T}he predominance of ineffective {A}zorhizobium strains in the nodules of {Z}ornia roots, the large {B}radyrhizobium genetic diversity and the geographical genetic diversity pattern are explored.},
  keywords = {{B}radyrhizobium ; {A}zorhizobium ; {Z}ornia glochidiata ; {S}ymbioses ; {N}itrogen fixation ; {P}arasitism ; {AFLP} ; {P}hylogeny},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {32},
  numero = {6},
  pages = {387--399},
  ISSN = {0723-2020},
  year = {2009},
  DOI = {10.1016/j.syapm.2009.04.004},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057521},
}