@article{fdi:010057292,
  title = {{A}nti-inflammatory activity of {M}itraphylline isolated from {U}ncaria tomentosa bark},
  author = {{R}ojas-{D}uran, {R}. and {G}onzalez-{A}spajo, {G}. and {R}uiz-{M}artel, {C}. and {B}ourdy, {G}enevi{\`e}ve and {D}oroteo-{O}rtega, {V}. {H}. and {A}lban-{C}astillo, {J}. and {R}obert, {G}. and {A}uberger, {P}. and {D}eharo, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {U}ncaria tomentosa ({W}illd. ex {R}oem. & {S}chult.) {DC}. ({R}ubiaceae) is widely used by populations living in {S}outh {A}merica to treat many ailments associated with inflammatory disorders. {M}itraphylline was shown to be the major pentacyclic oxindolic alkaloid present in the bark chloroformic extract of this plant. {I}ts activity against cytokines involved in inflammation process was tested in a murine model in vivo. {M}aterials and methods: {M}ice received mitraphylline once a day for 3 days at 30 mg/kg/day by oral route. {T}hen, they were subjected to bacterial lipopolysaccharide ({LPS}) endotoxin (15 mg/kg) and the {LPS}-induced production of 16 different cytokines was determined by {E}lisa multiplex. {C}ontrol group received dexamethasone orally at 2 mg/kg/day. {T}oxicity on {K}565 cells and murine peritoneal macrophages. in vitro, at doses up to 100 mu {M} was monitored by {XTT}-colorimetric assay. {R}esults and conclusions: {F}or the first time mitraphylline was tested in vivo against a large range of cytokines that play a crucial role in inflammation. {M}itraphylline inhibited around 50% of the release of interleukins 1 alpha, 1 beta, 17, and {TNF}-alpha. {T}his activity was similar to dexamethasone. {I}t also reduced almost 40% of the production of interleukin 4 ({IL}-4) while the corticoid did not. {L}astly it did not show any toxicity on {K}565 cells nor murine macrophages at doses up to 100 {MM}.},
  keywords = {{I}nflammation ; {U}ncaria tomentosa ; {P}entacyclic oxindolic alkaloids ; {T}raditional medicine ; {TNF}-alpha ; {M}itraphylline},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {143},
  numero = {3},
  pages = {801--804},
  ISSN = {0378-8741},
  year = {2012},
  DOI = {10.1016/j.jep.2012.07.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057292},
}