@article{fdi:010057284,
  title = {{E}ffects of cryopreservation of {P}haseolus vulgaris {L}. seeds on early stages of germination},
  author = {{C}ejas, {I}. and {V}ives, {K}. and {L}audat, {T}. and {G}onzalez-{O}lmedo, {J}. and {E}ngelmann, {F}lorent and {M}artinez-{M}ontero, {M}. {E}. and {L}orenzo, {J}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this work, we studied the effects of cryopreservation on various parameters of early stages of germination of {P}haseolus vulgaris seeds (0, 7 and 14 days). {P}ercentages of germination, fresh mass of different plant parts, levels of chlorophyll pigments (a, b, total), malondialdehyde, other aldehydes, phenolics (cell wall-linked, free, and total) and protein were determined. {N}o phenotypic changes were observed visually in seedlings recovered from cryopreserved seeds. {H}owever, several significant effects of seed liquid nitrogen exposure were recorded at the biochemical level. {T}here was a significant negative effect of cryopreservation on shoot protein content, which decreased from 3.11 mg g(-1) fresh weight for non-cryopreserved controls to 0.44 mg g(-1) fresh shoot weight for cryopreserved seeds. {O}n the other hand, cryopreservation significantly increased levels of other aldehydes than malondialdehyde in shoots at day 7, from 56.47 mu mol g(-1) for non-cryopreserved controls to 253.19 mu mol g(-1) fresh shoot weight for cryopreserved samples. {L}iquid nitrogen exposure significantly reduced phenolics contents (free, cell-wall linked, total) in roots at day 7 after onset of germination. {I}n general, roots were more affected by cryostorage compared with other plant parts, while leaves were the least affected. {T}he effects of seed cryopreservation seem to decline progressively along with seedling growth.},
  keywords = {{C}ommon bean ; {C}ryostorage ; {B}iochemical changes},
  booktitle = {},
  journal = {{P}lant {C}ell {R}eports},
  volume = {31},
  numero = {11},
  pages = {2065--2073},
  ISSN = {0721-7714},
  year = {2012},
  DOI = {10.1007/s00299-012-1317-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057284},
}