@article{fdi:010057264,
  title = {{E}lectroporation-mediated genetic vaccination for antigen mapping : application to {P}lasmodium falciparum {VAR}2{CSA} protein},
  author = {{B}ordbar, {B}. and {G}nidehou, {S}{\'e}dami and {T}uikue {N}dam, {N}icaise and {D}oritchamou, {J}. and {M}oussiliou, {A}. and {Q}uiviger, {M}. and {D}eloron, {P}hilippe and {S}cherman, {D}. and {B}igey, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}enetic vaccination, consisting in delivering a genetically engineered plasmid {DNA} by a non-viral vector or technique into a tissue, is currently of great interest. {N}ew delivery technique including {DNA} transfer by electroporation recently greatly improved the potency of this concept. {B}ecause it avoids the step of producing a recombinant protein, it is particularly of use in studying the immunogenic properties of large proteins. {H}ere we describe the use of electroporation mediated {DNA} immunization to identify important protective epitopes from the large {VAR}2{CSA} protein from {P}lasmodium falciparum implicated in the pathology of placental malaria. {I}mmunizing mice and rabbit with {DNA} plasmids encoding different fragments of {VAR}2{CSA} leads to high titer antisera. {M}oreover an {N}-terminal region of the protein was found to induce protective functional antibodies.},
  keywords = {{E}lectrotransfer ; {P}lasmid electroporation ; {G}enetic immunization ; {P}lacental malaria},
  booktitle = {},
  journal = {{B}ioelectrochemistry},
  volume = {87},
  numero = {{SI}},
  pages = {132--137},
  ISSN = {1567-5394},
  year = {2012},
  DOI = {10.1016/j.bioelechem.2011.12.009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057264},
}