@article{fdi:010057243,
  title = {{M}osquito feeding assays to determine the infectiousness of naturally infected {P}lasmodium falciparum gametocyte carriers},
  author = {{B}ousema, {T}. and {D}inglasan, {R}. {R}. and {M}orlais, {I}sabelle and {G}ouagna, {L}ouis-{C}l{\'e}ment and van {W}armerdam, {T}. and {A}wono-{A}mbene, {P}. {H}. and {B}onnet, {S}. and {D}iallo, {M}. and {C}oulibaly, {M}. and {T}chuinkam, {T}. and {M}ulder, {B}. and {T}argett, {G}. and {D}rakeley, {C}. and {S}utherland, {C}. and {R}obert, {V}incent and {D}oumbo, {O}. and {T}oure, {Y}. and {G}raves, {P}. {M}. and {R}oeffen, {W}. and {S}auerwein, {R}. and {B}irkett, {A}. and {L}ocke, {E}. and {M}orin, {M}. and {W}u, {Y}. {M}. and {C}hurcher, {T}. {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction: {I}n the era of malaria elimination and eradication, drug-based and vaccine-based approaches to reduce malaria transmission are receiving greater attention. {S}uch interventions require assays that reliably measure the transmission of {P}lasmodium from humans to {A}nopheles mosquitoes. {M}ethods: {W}e compared two commonly used mosquito feeding assay procedures: direct skin feeding assays and membrane feeding assays. {T}hree conditions under which membrane feeding assays are performed were examined: assays with i) whole blood, ii) blood pellets resuspended with autologous plasma of the gametocyte carrier, and iii) blood pellets resuspended with heterologous control serum. {R}esults: 930 transmission experiments from {C}ameroon, {T}he {G}ambia, {M}ali and {S}enegal were included in the analyses. {D}irect skin feeding assays resulted in higher mosquito infection rates compared to membrane feeding assays (odds ratio 2.39, 95% confidence interval 1.94-2.95) with evident heterogeneity between studies. {M}osquito infection rates in membrane feeding assays and direct skin feeding assays were strongly correlated (p < 0.0001). {R}eplacing the plasma of the gametocyte donor with malaria naive control serum resulted in higher mosquito infection rates compared to own plasma ({OR} 1.92, 95% {CI} 1.68-2.19) while the infectiousness of gametocytes may be reduced during the replacement procedure ({OR} 0.60, 95% {CI} 0.52-0.70). {C}onclusions: {D}espite a higher efficiency of direct skin feeding assays, membrane feeding assays appear suitable tools to compare the infectiousness between individuals and to evaluate transmission-reducing interventions. {S}everal aspects of membrane feeding procedures currently lack standardization; this variability makes comparisons between laboratories challenging and should be addressed to facilitate future testing of transmission-reducing interventions.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {7},
  numero = {8},
  pages = {e42821},
  ISSN = {1932-6203},
  year = {2012},
  DOI = {10.1371/journal.pone.0042821},
  URL = {https://www.documentation.ird.fr/hor/fdi:010057243},
}