@article{fdi:010057071, title = {{G}enome-wide analysis of micro{RNA}s in rubber tree ({H}evea brasiliensis {L}.) using high-throughput sequencing}, author = {{L}ertpanyasampatha, {M}. and {G}ao, {L}. and {K}ongsawadworakul, {P}. and {V}iboonjun, {U}. and {C}hrestin, {H}erv{\'e} and {L}iu, {R}. {Y}. and {C}hen, {X}. {M}. and {N}arangajavana, {J}.}, editor = {}, language = {{ENG}}, abstract = {{M}icro{RNA}s (mi{RNA}s) are short {RNA}s with essential roles in gene regulation in various organisms including higher plants. {I}n contrast to the vast information on mi{RNA}s from many economically important plants, almost nothing has been reported on the identification or analysis of mi{RNA}s from rubber tree ({H}evea brasiliensis {L}.), the most important natural rubber-producing crop. {T}o identify mi{RNA}s and their target genes in rubber tree, high-throughput sequencing combined with a computational approach was performed. {F}our small {RNA} libraries were constructed for deep sequencing from mature and young leaves of two rubber tree clones, {PB} 260 and {PB} 217, which provide high and low latex yield, respectively. 115 mi{RNA}s belonging to 56 known mi{RNA} families were identified, and northern hybridization validated mi{RNA} expression and revealed developmental stage-dependent and clone-specific expression for some mi{RNA}s. {W}e took advantage of the newly released rubber tree genome assembly and predicted 20 novel mi{RNA}s. {F}urther, computational analysis uncovered potential targets of the known and novel mi{RNA}s. {P}redicted target genes included not only transcription factors but also genes involved in various biological processes including stress responses, primary and secondary metabolism, and signal transduction. {I}n particular, genes with roles in rubber biosynthesis are predicted targets of mi{RNA}s. {T}his study provides a basic catalog of mi{RNA}s and their targets in rubber tree to facilitate future improvement and exploitation of rubber tree.}, keywords = {mi{RNA}s ; {R}ubber tree ; mi{R}396 ; mi{R}172 ; {R}ubber elongation factor ; {H}evamine {A}}, booktitle = {}, journal = {{P}lanta}, volume = {236}, numero = {2}, pages = {437--445}, ISSN = {0032-0935}, year = {2012}, DOI = {10.1007/s00425-012-1622-1}, URL = {https://www.documentation.ird.fr/hor/fdi:010057071}, }