@article{fdi:010056914,
  title = {{H}ybridisation of picoeukaryotes by eubacterial probes is widespread in the marine environment},
  author = {{B}iegala, {I}sabelle and {C}uttle, {M}. and {M}ary, {I}. and {Z}ubkov, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ost general and group-specific eubacterial probes hybridised picoeukaryotes in coastal waters ({B}rittany, {F}rance) and cultures of the dominant picoeukaryotes from this environment ({M}icromonas pusilla and {P}elagomonas calceolata). {T}his is either because they matched the 16{S} r{RNA} from organelles or because of the presence of symbiotic or antagonist intracellular bacteria. {T}he general eubacterial probe ({EUB}338) hybridised 84% of the picoeukaryotes, while the group-specific probes hybridised 3, 16, 10 and 34% of the picoeukaryotes for cyanobacteria ({CYA}664), alpha-proteobacteria ({ALF}968), gamma-proteobacteria ({GAM}42a) and {C}ytopbaga-{F}lavo-{B}acteria ({CF}319), respectively. {T}he results show that the hybridisation of eukaryote 16{S} r{RNA} by prokaryote probes can lead to significant errors in prokaryote counts, in particular for less well-represented groups such as cyanobacteria, with errors of 17% in the studied sample. {I}n addition, we revealed for the first time at this scale that up to 44% of the picoeukaryotes contained intracellular prokaryotes. {T}his finding might have serious implications for understanding the functioning of the microbial loop. {F}inally, because {SSU} r{RNA} databases have significantly been extended in recent years, we showed that the probe {PLA}886, which targets {P}lanctomycete 16{S} r{RNA}, labelled 87%. of the picoeukaryotes by hybridising their 18{S} r{RNA}. {C}onsequently, the design of this probe should be refined for future studies, and the presence of similar changes in probe specificity should be checked regularly when using hybridisation-based techniques.},
  keywords = {picoeukaryotes ; bacteria ; hybridisation ; diversity ; symbiont},
  booktitle = {},
  journal = {{A}quatic {M}icrobial {E}cology},
  volume = {41},
  numero = {3},
  pages = {293--297},
  ISSN = {0948-3055},
  year = {2005},
  DOI = {10.3354/ame041293},
  URL = {https://www.documentation.ird.fr/hor/fdi:010056914},
}