Arrighi Jean-François auteur aut IRD Cartieaux Fabienne auteur aut IRD Brown S. C. auteur aut IRD Rodier-Goud M. auteur aut IRD Boursot Marc auteur aut IRD Fardoux Joël auteur aut IRD Patrel Delphine auteur aut IRD Gully Djamel auteur aut IRD Fabre S. auteur aut IRD Chaintreuil Clémence auteur aut IRD Giraud Eric auteur aut IRD text journalArticle eng text/pdf born digital access Research on the nitrogen-fixing symbiosis has been focused, thus far, on two model legumes, Medicago truncatula and Lotus japonicus, which use a sophisticated infection process involving infection thread formation. However, in 25% of the legumes, the bacterial entry occurs more simply in an intercellular fashion. Among them, some Aeschynomene spp. are nodulated by photosynthetic Bradyrhizohium spp. that do not produce Nod factors. This interaction is believed to represent a living testimony of the ancestral state of the rhizobium legume symbiosis. To decipher the mechanisms of this Nod-independent process, we propose Aeschynomene evenia as a model legume because it presents all the characteristics required for genetic and molecular analysis. It is a short-perennial and autogamous species, with a diploid and relatively small genome (2n = 20; 460 Mb/IC). A. evenia `IRFL6945' is nodulated by the well-characterized photosynthetic Bradyrhizobium sp. strain ORS278 and is efficiently transformed by Agrobacterizon rhizogenes. Aeschynomene evenia is genetically homozygous but polymorphic accessions were found. A manual hybridization procedure has been set up, allowing directed crosses. Therefore, it should be relatively straightforward to unravel the molecular determinants of the Nod-independent process in A. evenia. This should shed new light on the evolution of rhizobium legume symbiosis and could have important agronomic implications. specialized 084 25 7 851-861 2012 0894-0282 https://www.documentation.ird.fr/hor/fdi:010055970 http://dx.doi.org/10.1094/MPMI-02-12-0045-TA 0894-0282 [F B010055970] https://www.documentation.ird.fr/hor/fdi:010055970 https://www.documentation.ird.fr/intranet/publi/2012/07/010055970.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2012-07-24 2017-08-23 fdi:010055970 fre