@article{fdi:010055887,
  title = {{I}n-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches},
  author = {{L}orieux, {M}athias and {B}lein, {M}. and {L}ozano, {J}. and {B}ouniol, {M}. and {D}roc, {G}. and {D}ievart, {A}. and {P}erin, {C}. and {M}ieulet, {D}. and {L}anau, {N}. and {B}es, {M}. and {R}ouviere, {C}. and {G}ay, {C}. and {P}iffanelli, {P}. and {L}armande, {P}ierre and {M}ichel, {C}. and {B}arnola, {I}. and {B}iderre-{P}etit, {C}. and {S}allaud, {C}. and {P}erez, {P}. and {B}ourgis, {F}abienne and {G}hesqui{\`e}re, {A}lain and {G}antet, {P}ascal and {T}ohme, {J}. and {M}orel, {J}. {B}. and {G}uiderdoni, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar {N}ipponbare of rice ({O}ryza sativa {L}.), called {O}ryza {T}ag {L}ine ({OTL}). {S}ixteen thousand eight hundred and fourteen {T}-{DNA} and 12 410 {T}os17 discrete insertion sites have been characterized in these lines. {W}e estimate that 8686 predicted gene intervalsi.e. one-fourth to one-fifth of the estimated rice nontransposable element gene complementare interrupted by sequence-indexed {T}-{DNA} (6563 genes) and/or {T}os17 (2755 genes) inserts. {S}ix hundred and forty-three genes are interrupted by both {T}-{DNA} and {T}os17 inserts. {H}igh quality of the sequence indexation of the {T}2 seed samples was ascertained by several approaches. {F}ield evaluation under agronomic conditions of 27 832 {OTL} has revealed that 18.2% exhibit at least one morphophysiological alteration in the {T}1 progeny plants. {S}creening 10 000 lines for altered response to inoculation by the fungal pathogen {M}agnaporthe oryzae allowed to observe 71 lines (0.7%) developing spontaneous lesions simulating disease mutants and 43 lines (0.4%) exhibiting an enhanced disease resistance or susceptibility. {W}e show here that at least 3.5% (four of 114) of these alterations are tagged by the mutagens. {T}he presence of allelic series of sequence-indexed mutations in a gene among {OTL} that exhibit a convergent phenotype clearly increases the chance of establishing a linkage between alterations and inserts. {T}his convergence approach is illustrated by the identification of the rice ortholog of {A}t{PHO}2, the disruption of which causes a lesion-mimic phenotype owing to an over-accumulation of phosphate, in nine lines bearing allelic insertions.},
  keywords = {forward genetics ; insertion mutagenesis ; reverse genetics ; rice},
  booktitle = {},
  journal = {{P}lant {B}iotechnology {J}ournal},
  volume = {10},
  numero = {5},
  pages = {555--568},
  ISSN = {1467-7644},
  year = {2012},
  DOI = {10.1111/j.1467-7652.2012.00689.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055887},
}