@article{fdi:010055833,
  title = {{I}nsecticide resistance in the dengue vector {A}edes aegypti from {M}artinique : distribution, mechanisms and relations with environmental factors},
  author = {{M}arcombe, {S}{\'e}bastien and {M}athieu, {R}. {B}. and {P}ocquet, {N}icolas and {R}iaz, {M}. {A}. and {P}oupardin, {R}. and {S}elior, {S}. and {D}arriet, {F}r{\'e}d{\'e}ric and {R}eynaud, {S}. and {Y}ebakima, {A}ndr{\'e} and {C}orbel, {V}incent and {D}avid, {J}. {P}. and {C}handre, {F}abrice},
  editor = {},
  language = {{ENG}},
  abstract = {{D}engue is an important mosquito borne viral disease in {M}artinique {I}sland ({F}rench {W}est {I}ndies). {T}he viruses responsible for dengue are transmitted by {A}edes aegypti, an indoor day-biting mosquito. {T}he most effective proven method for disease prevention has been by vector control by various chemical or biological means. {U}nfortunately insecticide resistance has already been observed on the {I}sland and recently showed to significantly reduce the efficacy of vector control interventions. {I}n this study, we investigated the distribution of resistance and the underlying mechanisms in nine {A}e. aegypti populations. {S}tatistical multifactorial approach was used to investigate the correlations between insecticide resistance levels, associated mechanisms and environmental factors characterizing the mosquito populations. {B}ioassays revealed high levels of resistance to temephos and deltamethrin and susceptibility to {B}ti in the 9 populations tested. {B}iochemical assays showed elevated detoxification enzyme activities of monooxygenases, carboxylesterases and glutathione {S}-tranferases in most of the populations. {M}olecular screening for common insecticide target-site mutations, revealed the presence of the "knockdown resistance' {V}1016{I} {K}dr mutation at high frequency (> 87%). {R}eal time quantitative {RT}-{PCR} showed the potential involvement of several candidate detoxification genes in insecticide resistance. {P}rincipal {C}omponent {A}nalysis ({PCA}) performed with variables characterizing {A}e. aegypti from {M}artinique permitted to underline potential links existing between resistance distribution and other variables such as agriculture practices, vector control interventions and urbanization. {I}nsecticide resistance is widespread but not homogeneously distributed across {M}artinique. {T}he influence of environmental and operational factors on the evolution of the resistance and mechanisms are discussed.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {7},
  numero = {2},
  pages = {e30989},
  ISSN = {1932-6203},
  year = {2012},
  DOI = {10.1371/journal.pone.0030989},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055833},
}