@article{fdi:010055823,
  title = {{C}ellular and molecular analyses of coffee resistance to {H}emileia vastatrix and nonhost resistance to {U}romyces vignae in the resistance-donor genotype {HDT}832/2},
  author = {{D}iniz, {I}. and {T}alhinhas, {P}. and {A}zinheira, {H}. {G}. and {V}arzea, {V}. and {M}edeira, {C}. and {M}aia, {I}. and {P}etitot, {A}nne-{S}ophie and {N}icole, {M}ichel and {F}ernandez, {D}iana and {D}o {C}eu {S}ilva, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n {A}rabica coffee breeding, some of the most used sources of resistance to leaf rust ({H}emileia vastatrix) are natural {C}offea arabica x canephora hybrids ("{H}ibrido de {T}imor"). {T}o decipher the cellular and molecular nature of that resistance, leaves of genotype {HDT}832/2, were challenged with {H}. vastatrix race {II}, and monitored using light microscopy and {RT}-q{PCR} expression analysis of genes involved in plant immunity mechanisms (receptor-like kinase, {WRKY} transcription factor 1, phenylalanine ammonia-lyase, chalcone synthase, 13-lipoxygenase, glycosyltransferase, pathogenesis related {PR}1b and {PR}10). {T}hese were compared to the nonhost resistance responses of {HDT}832/2 to the infection by the cowpea rust fungus ({U}romyces vignae). {H}. vastatrix ceased growth more frequently after stomata penetration, forming few haustoria, inducing a hypersensitive-like response, phenol accumulation and haustorium encasement with callose. {U}. vignae could enter stomata but failed to form haustoria, while inducing hypersensitive-like responses and phenol accumulation. {I}n host and nonhost interactions, activation of genes involved in signalling coincided with the differentiation of appressoria, and cellular responses (hypersensitive-like responses and accumulation of phenolic compounds) were recorded from the full appressorium or penetration hypha stages onwards. {S}imilarly, a gene related to the {JA} pathway was first activated at the penetration hypha stage for both interactions, while genes related to the {SA} pathway were only activated in the host interaction, the latter being the single clear difference between host and nonhost interactions. {T}he cellular and molecular resistance responses of {HDT}832/2 to these rust fungi suggest that common immunity components are shared between host and nonhost resistance, which may explain the longer durability of this resistance.},
  keywords = {{B}asal immunity ; {C}offea arabica ; {C}offea canephora ; {C}offee leaf rust ; {D}efence gene expression ; {H}ypersensitive response},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {P}lant {P}athology},
  volume = {133},
  numero = {1},
  pages = {141--157},
  ISSN = {0929-1873},
  year = {2012},
  DOI = {10.1007/s10658-011-9925-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055823},
}